We narrowed to 8,541 results for: sgrna

1. pX330-NQL007-NANOG-sgRNA

   Plasmid

   #175555

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiCRISPR - Amplicon, JAK2 sgRNA 1

   Plasmid

   #70679

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. SauCas9KKH CCR5-nicking sgRNA

   Plasmid

   #169863

   Purpose

   SauCas9KKH nicking sgRNA for CCR5

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9KKH CCR5-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pM117: VEGFA array presgRNA

   Plasmid

   #166869

   Purpose

   U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   Human VEGFA targeting array presgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

   Plasmid

   #149656

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC57-Sa sgRNA expression vector

   Plasmid

   #107720

   Purpose

   For in vitro transcription of Sa sgRNA from the T7 promoter.

   Depositor

   Xingxu Huang

   Article

   Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAN-PBAD-sgRNA-A2T

   Plasmid

   #62258

   Purpose

   expression of A2T sgRNA from the arabinose-inducible promoter

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   A2T sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cas9 ROSA sgRNA mCherry

   Plasmid

   #155280

   Purpose

   Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker

   Depositor

   Martin Carroll

   Insert

   spCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCRISPR - Amplicon, JAK2 sgRNA 2

   Plasmid

   #70660

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. px335-sgRNA-EF(Cas9n)-Eif4a1-L

    Plasmid

    #122346

    Purpose

    Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Eif4a1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138316

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAN-PBAD-sgRNA-A1T

    Plasmid

    #62256

    Purpose

    expression of A1T sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A1T sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAN-PBAD-sgRNA-A4NT

    Plasmid

    #62261

    Purpose

    expression of A4NT sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A4NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Icam 2 SaCas9 YAP sgRNA3

    Plasmid

    #99736

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO1-puro-U6-sgRNA-LRTM2

    Plasmid

    #69237

    Purpose

    U6 driven SpCas9 sgRNA expression for LRTM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    LRTM2 sgRNA (LRTM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAN-PBAD-sgRNA-A3T

    Plasmid

    #62260

    Purpose

    expression of A3T sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A3T sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAN-PBAD-sgRNA-A3NT

    Plasmid

    #62259

    Purpose

    expression of A3NT sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A3NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mU6-sgRNA-CLTA-mCherry

    Plasmid

    #240637

    Purpose

    Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    puro-T2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pdU6-2_sgRNA1-5-HT2AR-isoformD

    Plasmid

    #221823

    Purpose

    Plasmid to express gRNA1 (tccttctggcgcaaacacgg) for editing at the end of Drosophila 5-HT2AR isoform D coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    d5-HT2AR isoform D gRNA1 (5-HT2 Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6 promoter

    Available since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pdU6-2_sgRNA2-5-HT2AR-isoformD

    Plasmid

    #221824

    Purpose

    Plasmid to express gRNA2 (ctgaagacataattacgtgg) for editing at the end of Drosophila 5-HT2AR isoform D coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    d5-HT2AR isoform D gRNA2 (5-HT2 Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    zebrafish U6 promoter (U6b)

    Available since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pdU6-2_sgRNA1-5-HT2AR-isoformBFH

    Plasmid

    #221825

    Purpose

    Plasmid to express gRNA1 (cgctatcggtctgtgacaga) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    d5-HT2AR isoforms BFH gRNA1 (5-HT2 Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    zebrafish U6 promoter (U6b)

    Available since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pdU6-2_sgRNA2-5-HT2AR-isoformBFH

    Plasmid

    #221826

    Purpose

    Plasmid to express gRNA2 (ggaaaagccgctaattacag) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    d5-HT2AR isoforms BFH gRNA2 (5-HT2 Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6 promoter

    Available since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LentiCRISPR-B_2xsgRNAs_UPF3A/UPF3B (pAVA3129)

    Plasmid

    #239329

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting UPF3A and UPF3B

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA1 targting UPF3A and 7SK-driven sgRNA2 targeting UPF3B (UPF3B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LentiCRISPR-B_sgRNA-RMP64 (pAVA3500)

    Plasmid

    #239237

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

    Plasmid

    #239240

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP24
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LentiCRISPR-B_sgRNA-RMP24 (pAVA3555)

    Plasmid

    #239242

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP24
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiCRISPR-B_sgRNA-RMP24 (pAVA3563)

    Plasmid

    #239258

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP24
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LentiCRISPR-B_sgRNA-RMP64 (pAVA3572)

    Plasmid

    #239259

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

    Plasmid

    #239260

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

    Plasmid

    #239261

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LentiCRISPR-B_2xsgRNAs-RPPH1 (pAVA3585)

    Plasmid

    #239262

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPPH1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV-U6-sgRNA-EF1a-LibVec

    Plasmid

    #239591

    Purpose

    AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Lenti-U6-sgRNA-BFP-Puro

    Plasmid

    #229014

    Purpose

    Perturb Seq lentiviral sgRNA vector

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLentiCRISPR V2 Neo PTEN sgRNA5

    Plasmid

    #233246

    Purpose

    Knock out of murine PTEN

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    PTEN (Pten Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLentiCRISPR V2 Neo PTEN sgRNA2

    Plasmid

    #233245

    Purpose

    Knock out of murine PTEN

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    PTEN (Pten Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. esgRNA_NbATML1-2pro and NbATML1-1pro

    Plasmid

    #231155

    Purpose

    T-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pM148: pAAV.CMV-Cas13e-NT sgRNA

    Plasmid

    #203446

    Purpose

    Plasmid expressing active RfxCas13d with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LsgRNA-FXR1-KH1-gRNA

    Plasmid

    #225483

    Purpose

    Express gRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    gRNA for editing FXR1-KH1 (FXR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mPLD3-sgRNA-Cas9-mcherry

    Plasmid

    #199700

    Purpose

    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. sgRNA scaffold-spacer-mouse U6

    Plasmid

    #214698

    Purpose

    provide "sgRNA scaffold-spacer-mouse U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-mouse U6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge