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We narrowed to 5,231 results for: codon optimized

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Showing: 1251 - 1300 of 5231 results

  2. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  4. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available Since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  7. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  9. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

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  12. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  15. pTH849-gst-optec

    Plasmid
    #89590
    Purpose
    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89590-plasmid-map-sequence-id-206038

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  16. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  17. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid
    #177209
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177209-plasmid-map-sequence-id-348605

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  18. pHH0103 C20orf67 WW domain

    Plasmid
    #104273
    Purpose
    Bacterial expression of WW domain from C20orf67
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    C20orf67 WW domain (PCIF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104273-plasmid-map-sequence-id-203480

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  19. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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  20. pBS-L1PA1CH_blast rescue

    Plasmid
    #69611
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Tags
    blast rescue cassette
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69611-plasmid-map-sequence-id-123344

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  21. PKMYT1

    Plasmid
    #39061
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PKMYT1 (PKMYT1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid
    #177208
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available Since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    177208-plasmid-map-sequence-id-356946

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  23. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid
    #195487
    Purpose
    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    C61T, C262V
    Promoter
    T7
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195487-plasmid-map-sequence-id-393190

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  24. p426TEF-LptTPS-A

    Plasmid
    #89468
    Purpose
    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production
    Depositor
    Jing-Ke Weng
    Insert
    LptTPS-A
    Tags
    none
    Expression
    Yeast
    Promoter
    pBR322 origin of replication for propagation in E…
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89468-plasmid-map-sequence-id-176087

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  25. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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  26. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  27. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  28. pHH0103 NEDD4 WW domain #3

    Plasmid
    #104210
    Purpose
    Bacterial expression of WW domain #3 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #3 (NEDD4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104210-plasmid-map-sequence-id-204199

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  29. pHH0103 NEDD4 WW domain #1

    Plasmid
    #104208
    Purpose
    Bacterial expression of WW domain #1 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #1 (NEDD4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104208-plasmid-map-sequence-id-204367

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  30. pHH0103 NEDD4 WW domain #4

    Plasmid
    #104211
    Purpose
    Bacterial expression of WW domain #4 from NEDD4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4 WW domain #4 (NEDD4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104211-plasmid-map-sequence-id-201499

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  31. SPR

    Plasmid
    #39161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. PBRM1

    Plasmid
    #39013
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. PBRM1

    Plasmid
    #39027
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    polybromo 1 [isoform a] (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. PBRM1

    Plasmid
    #38999
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. PBRM1

    Plasmid
    #39028
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. pHH0103 WWP2 WW domain #1

    Plasmid
    #104212
    Purpose
    Bacterial expression of WW domain #1 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #1 (WWP2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104212-plasmid-map-sequence-id-204807

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  37. pHH0103 WWP2 WW domain #3

    Plasmid
    #104214
    Purpose
    Bacterial expression of WW domain #3 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #3 (WWP2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104214-plasmid-map-sequence-id-204291

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  38. pHH0103 WWP2 WW domain #2

    Plasmid
    #104213
    Purpose
    Bacterial expression of WW domain #2 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #2 (WWP2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104213-plasmid-map-sequence-id-204203

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  39. pHH0103 WWP2 WW domain #4

    Plasmid
    #104215
    Purpose
    Bacterial expression of WW domain #4 from WWP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP2 WW domain #4 (WWP2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104215-plasmid-map-sequence-id-204195

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  40. pHH0103 SMURF2 WW domain #3

    Plasmid
    #104226
    Purpose
    Bacterial expression of WW domain #3 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #3 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104226-plasmid-map-sequence-id-204295

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  41. pHH0103 SMURF2 WW domain #2

    Plasmid
    #104225
    Purpose
    Bacterial expression of WW domain #2 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #2 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104225-plasmid-map-sequence-id-204192

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  42. pHH0103 SMURF2 WW domain #1

    Plasmid
    #104224
    Purpose
    Bacterial expression of WW domain #1 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #1 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104224-plasmid-map-sequence-id-201513

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  43. PBRM1

    Plasmid
    #39030
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    polybromo 1 [isoform a] (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  44. pHH0103 PLEKHA5 WW domain #2

    Plasmid
    #104259
    Purpose
    Bacterial expression of WW domain #2 from PLEKHA5
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PLEKHA5 WW domain #2 (PLEKHA5 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104259-plasmid-map-sequence-id-202581

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  45. pHH0103 WBP4 WW domain #2

    Plasmid
    #104253
    Purpose
    Bacterial expression of WW domain #2 from WBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WBP4 WW domain #2 (WBP4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104253-plasmid-map-sequence-id-204383

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  46. pET-29b(+)_His-CBD-EmEndoF3

    Plasmid
    #218194
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3
    Depositor
    Florence Pojer
    Insert
    EndoF3
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-329
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218194-plasmid-map-sequence-id-431796

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  47. pET-29b(+)_notufA-EmEndoF2-CBD-His

    Plasmid
    #218197
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2
    Depositor
    Florence Pojer
    Insert
    EndoF2
    Tags
    CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-335
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218197-plasmid-map-sequence-id-431793

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  48. pET-29b(+)_His-CBD-EmEndoF1

    Plasmid
    #218195
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1
    Depositor
    Florence Pojer
    Insert
    EndoF1
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 51-339
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218195-plasmid-map-sequence-id-431792

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  49. pET3-ARL15(CO)

    Plasmid
    #201879
    Purpose
    Used for bacterial expression of human protein ARL15
    Depositor
    Richard Kahn
    Insert
    ARL15 (ARL15 Human)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201879-plasmid-map-sequence-id-403808

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  50. pHH0103 TCERG1L WW domain #2

    Plasmid
    #104281
    Purpose
    Bacterial expression of WW domain #2 from TCERG1L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    TCERG1L WW domain #2 (TCERG1L Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104281-plasmid-map-sequence-id-202574

    Enlarge

Showing: 1251 - 1300 of 5231 results