We narrowed to 1,535 results for: MK

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E. coli 16S toehold switch sensor

Plasmid

#110698

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. coli 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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B1 Integrin

Plasmid

#186137

Purpose

For expression of recombinant beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP and untranslated His6 after stop codon

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1 Trx-Bx

Plasmid

#106290

Purpose

Expresses Trx-Bx in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Trx-BX

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Amida

Plasmid

#15362

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
B. fragilis 16S toehold switch sensor

Plasmid

#110696

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. fragilis 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-1x

Plasmid

#124690

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo5(N140A)

Plasmid

#157806

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5(N140A)

Tags

His6 and MBP

Expression

Bacterial

Mutation

N140A

Promoter

pTAC

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P32

Plasmid

#120043

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z25

Plasmid

#120068

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-Start-1351

Plasmid

#46075

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-Start-1351 (Map7 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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FKBP-YPet-RanGAP*

Plasmid

#175245

Purpose

Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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CyPet-FKBP-E2

Plasmid

#175244

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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B. fragilis mRNA toehold switch sensor

Plasmid

#110706

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. fragilis species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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SB53

Plasmid

#21488

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Tags

HA

Expression

Mammalian

Mutation

aa1-65 deleted

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
His10-B1 Integrin

Plasmid

#186139

Purpose

For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP + uncleavable His10

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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C. difficile 16S toehold switch sensor

Plasmid

#110704

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

C. difficile 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PU.1-W215G LZRS

Plasmid

#44635

Depositor

Ellen Rothenberg

Article

Anderson et al Immunity. 2002 Feb;16(2):285-96.

Insert

PU.1 W215G (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

215 mutation (W to G)

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
His10-PS-SNAPf-9E (XSB526)

Plasmid

#78514

Purpose

Construct for expressing His10 - SNAPf -9E in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

SNAPf

Tags

His10 followed by a prescission protease claevage…

Expression

Bacterial

Mutation

9E added to the C-terminal of SNAPf

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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