We narrowed to 14,012 results for: Ung;

1. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBRα-β flap (831-1057)

   Plasmid

   #53734

   Purpose

   Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   alpha NTD-Beta flap fusion
   

   Expression

   Bacterial

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NAX_CAGGS_CAS9_mcherry

   Plasmid

   #167862

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CAG-FLEX-Chrimson-TdTomato

   Plasmid

   #209202

   Purpose

   Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics

   Depositor

   Larry Zweifel

   Article

   Soden et al Nat Neurosci. 2020 Aug;23(8):968-980. do

   Insert

   Chrimson
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. #1.4 pre-R-SynapShot-TagBFP

   Plasmid

   #214719

   Purpose

   Expresses red #1.4 pre-SynapShot and TagBFP

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #1.4 pre-R-SynapShot
   

   Use

   AAV

   Tags

   TagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. #1.4 post-R-SynapShot-Lyn-miRFP670

   Plasmid

   #214718

   Purpose

   Expresses red #1.4 post-SynapShot and Lyn-miRFP670

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #1.4 post-R-SynapShot
   

   Tags

   Lyn-miRFP670

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lox-Stop-Lox TOPO

   Plasmid

   #11584

   Purpose

   Cre-dependent activation of gene of interest by removing Stop sequences; Puro selection.

   Depositor

   Tyler Jacks

   Article

   Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

   Insert

   loxP-STOP-loxP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Fuw-dCas9-Tet1CD_IM

   Plasmid

   #84479

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD_IM
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-His-SCov2-nsp7

   Plasmid

   #154757

   Purpose

   Expression of SARS-CoV-2 nsp7

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMN-334

    Plasmid

    #195468

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-His-SCov2-nsp8

    Plasmid

    #154758

    Purpose

    Expression of SARS-CoV-2 nsp8

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHBV-Luc

    Plasmid

    #71414

    Purpose

    To see the activity of HBV transcription using Luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

    Insert

    HBV core promoter, enhancer I, enhancer II and luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    HBV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

    Plasmid

    #104116

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    QuasAr3-Citrine
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human synapsin I

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Alpha1

    Plasmid

    #118044

    Purpose

    GoldenBraid2.0 Alpha 1 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-TET

    Plasmid

    #20909

    Purpose

    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Omega 1

    Plasmid

    #118046

    Purpose

    GoldenBraid2.0 Omega 1 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-Nkx2-1/Puro

    Plasmid

    #31271

    Depositor

    Monte Winslow, Tyler Jacks

    Article

    Winslow et al Nature. 2011 May 5. 473(7345):101-4.

    Insert

    Nkx2-1* (Nkx2-1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Omega 2

    Plasmid

    #118047

    Purpose

    GoldenBraid2.0 Omega 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Alpha2

    Plasmid

    #118045

    Purpose

    GoldenBraid2.0 Alpha 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCIneoRL-BCL2 3'UTR

    Plasmid

    #84597

    Purpose

    Renilla luciferase reporter containing BCL2 3' UTR

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    BCL2 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

22. B23(9-122)

    Plasmid

    #23142

    Depositor

    SeWon Suh

    Article

    Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

    Insert

    B23 core (NPM1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-8

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pLiLucB

    Plasmid

    #31596

    Depositor

    Michele Calos

    Article

    Woodard et al PLoS One. 2010 . 5(6):e11367.

    Insert

    alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Fuw-dCas9-Dnmt3a_IM

    Plasmid

    #84478

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a_IM
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pC0040-LwaCas13a crRNA backbone

    Plasmid

    #103851

    Purpose

    Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-LwaCas13aDR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. #2.4 pre-G-SynapShot-TagBFP

    Plasmid

    #214721

    Purpose

    Expresses green #2.4 pre-SynapShot and TagBFP

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #2.4 pre-G-SynapShot
    

    Use

    AAV

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMN-333

    Plasmid

    #195467

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC018 - LshCas13a from Leptotrichia shahii

    Plasmid

    #91908

    Purpose

    Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LshCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMZ-180

    Plasmid

    #195428

    Purpose

    Transient, constitutive expression of synZiFTR: ZF5-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMN-013

    Plasmid

    #195417

    Purpose

    AAVS1 Integratable mCherry Reporter for synZiFTR ZF5

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge