159,332 results

1. pJL1-sfGFP-MOOR

   Plasmid

   #198212

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_MOOR
   

   Use

   Tags

   6x His Tag and MOOR glycosylation tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

   Plasmid

   #148859

   Purpose

   Mammalian Expression of HsXRN1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsXRN1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR_PGK_SNIPR_hsNotch3 LNR

   Plasmid

   #188338

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_Notch3-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR_PGK_SNIPR_FAP site with scFv

   Plasmid

   #188351

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3.2/V5-DEST-Noa1

   Plasmid

   #191969

   Purpose

   Overexpression of Nitric Oxide Associated 1 protein (NOA1), tagged with V5.

   Depositor

   Luca Scorrano

   Insert

   Noa1
   

   Use

   Synthetic Biology

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAdx-EF1-tdTomato

   Plasmid

   #73354

   Purpose

   Express tdTomato under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   EF1-tdTomato
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FRE5

   Plasmid

   #62377

   Purpose

   Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   dsRed and GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET14-Encap

   Plasmid

   #86405

   Purpose

   Expresses T. maritima encapsulin in E. coli

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Insert

   Encapsulin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p323-L10A-PATagRFP

    Plasmid

    #74172

    Purpose

    Fluorescent reporter for single ribosome tracking

    Depositor

    Robert Singer

    Article

    Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

    Insert

    ribosomal protein L10A (Rpl10a Rat)
    

    Use

    Lentiviral

    Tags

    PATagRFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TIGRE docking site targeting vector

    Plasmid

    #61580

    Purpose

    Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

    Plasmid

    #194063

    Purpose

    Mammalian expression of evoCjCas9 PEmax_deltaRnH

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-PEmax_dRnH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Ef1a-Con/Fon oG

    Plasmid

    #131778

    Purpose

    INTRSECT 2.0

    Depositor

    Karl Deisseroth

    Article

    Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

    Insert

    rabies glycoprotein - oG
    

    Use

    AAV; Intrsect

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF0589

    Plasmid

    #142653

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMK419 (GAL-OsTIR1(F74G))

    Plasmid

    #140656

    Purpose

    GAL-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    GAL-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Gamillus/ pcDNA3

    Plasmid

    #124837

    Purpose

    mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

    Insert

    Gamillus
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC18R6K-mini-Tn7T-Gm

    Plasmid

    #65022

    Purpose

    GmR mini-Tn7 vector, R6K replicon

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. EF.STAT3C.Ubc.GFP

    Plasmid

    #24983

    Purpose

    3rd generation lentiviral expression of constitutively active Stat3

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A662C, N664C (constitutively active)

    Promoter

    Available since

    May 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-myc-GLUT4-mCherry

    Plasmid

    #64049

    Purpose

    3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    GLUT4 (Slc2a4 Mouse)
    

    Use

    Lentiviral

    Tags

    c-Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. U6_crRNA_mCherry

    Plasmid

    #197609

    Purpose

    Expresses a Cas7-11 crRNA and mCherry

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    U6_crRNA_mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge