164,722 results

1. pAG1160 mCherry-FIREtag

   Plasmid

   #205164

   Purpose

   Expresses mCherry -FIREtag in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   mCherry-FIREtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEYFP-C1-Drp1

   Plasmid

   #45160

   Depositor

   Richard Youle

   Article

   Frank et al Dev Cell. 2001 Oct;1(4):515-25.

   Insert

   Drp1 (DNM1L Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

   Plasmid

   #172067

   Purpose

   Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP11x7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHJ12: CadC-VHH-Caffeine (No linker)

   Plasmid

   #108244

   Purpose

   Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLacO1

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL

   Plasmid

   #110739

   Purpose

   Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin and p10 promoters

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-neo largeTcDNA

   Plasmid

   #1780

   Purpose

   Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1-B-GRAM-W-NeoR

   Plasmid

   #211710

   Purpose

   Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Lentiviral

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGW-GOI-TurboID-GFP

   Plasmid

   #209379

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-ninein

   Plasmid

   #73519

   Purpose

   Encodes mouse ninein tagged with an EGFP tag at its N-terminus

   Depositor

   Michel Bornens

   Article

   Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

   Insert

   ninein (Nin Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD_Kan-LacI

    Plasmid

    #79826

    Purpose

    Arabinose induced expression of Lac repressor

    Depositor

    Katja Arndt

    Article

    Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

    Insert

    LacI
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    araBp

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGDP1 fosA

    Plasmid

    #112889

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    fosA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TS module

    Plasmid

    #26021

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Tension Sensor module
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid

    #125256

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2(H134R)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-forward

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3xFLAG CMV14 / BRAF-V600E

    Plasmid

    #131723

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    CMV

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. cacnb2a (rat) in pMT2 vector

    Plasmid

    #107424

    Purpose

    Expresses Calcium channel beta2a auxillary subunit in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

    Insert

    calcium channel beta2a auxillary subunit (Cacnb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

    Plasmid

    #194171

    Purpose

    Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-Associated Protein Tau 0N4R
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVET-IRES-GFP

    Plasmid

    #107139

    Purpose

    A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ki67-mNeonGreen-IRESpuro2

    Plasmid

    #183737

    Purpose

    Expresses Ki67-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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