19,646 results

1. pCDH-TMEM106b-mApple

   Plasmid

   #179385

   Purpose

   Lentiviral vector for expression of TMEM106b fused mApple

   Depositor

   Angus Johnston

   Article

   Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

   Insert

   TMEM106b - mApple
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLOX-TERT-iresTK

   Plasmid

   #12245

   Purpose

   2nd generation lentiviral vector for reversible immortalization of human primary cells

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   TERT, HSV1-TK (TERT Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   LoxP in 3' LTR.

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-GFP

   Plasmid

   #25999

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-GFP (H2BC21 Human, A. victoria)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. SIRT7 Flag

   Plasmid

   #13818

   Purpose

   Mammalian expression of human SIRT7 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT7 (SIRT7 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Missing exon 2

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGolt-mCherry / pGolt3-mCherry

   Plasmid

   #73297

   Purpose

   Visualization of Dendritic Golgi Satellite

   Depositor

   Michael Kreutz

   Article

   Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

   Inserts

   ER export signal of Scap
   ER export signal of Scap
   TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
   

   Tags

   ER export and mCherry

   Expression

   Mammalian

   Promoter

   CMV immediate early promoter

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6-MK1-EHMT2-V5

   Plasmid

   #31113

   Depositor

   Bernard Futscher

   Insert

   euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Stop codon mutated to Ala

   Available Since

   Aug. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKO.1 puro

   Plasmid

   #8452

   Purpose

   Retroviral vector for shRNA expression.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. Halo-Sec61-C-18

   Plasmid

   #123285

   Purpose

   Localization: Endoplasmic Reticulum

   Depositor

   Kevin McGowan

   Insert

   Sec61 (SEC61B Human)
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL

   Plasmid

   #196286

   Purpose

   35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV L antigenome encoding codon-optimized L (RdRp)
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mem-TurboID

    Plasmid

    #149409

    Purpose

    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    mem-TurboID
    

    Tags

    HA and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cherry-LacRep

    Plasmid

    #18985

    Depositor

    Mirek Dundr

    Article

    Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

    Insert

    LacI
    

    Tags

    AviTag, SV40 NLS, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV SYN flex PSAM4 GlyR IRES EGFP

    Plasmid

    #119741

    Purpose

    Cre-dependent chemogenetic inhibitor expression

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5 and AAV9

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. spNW80

    Plasmid

    #173487

    Purpose

    Expresses spNW80 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW80
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PT3.5/CMV-EGFRvIII

    Plasmid

    #20280

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    EGFRvII (EGFR Human)
    

    Use

    Transposon

    Mutation

    See depositor comments below.

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. TUBB5-Halo

    Plasmid

    #64691

    Purpose

    Expresses beta-tubulin labeled with Halo-tag

    Depositor

    Yasushi Okada

    Article

    Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

    Insert

    TUBB
    

    Tags

    Halo-tag

    Expression

    Mammalian

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMDR1-1202

    Plasmid

    #37627

    Depositor

    Kathleen Scotto

    Article

    Hu et al J Biol Chem. 2000 Jan 28;275(4):2979-85.

    Insert

    MDR1 promoter (ABCB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. GAPDH-CPG-TV

    Plasmid

    #70148

    Purpose

    a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRU294

    Plasmid

    #167689

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Salsa6f

    Plasmid

    #140188

    Purpose

    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

    Depositor

    Michael Cahalan

    Article

    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

    Insert

    Salsa6f
    

    Tags

    tdTomato-v5-GCaMP6f

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-EF1-DIO-tdTomato

    Plasmid

    #72254

    Purpose

    Express tdTomato under EF-1 promoter when Cre is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    tdTomato
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    elongation factor-1

    Available Since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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