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ABE8e-dimer

Plasmid

#138490

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-CRY2-OCRL

Plasmid

#79565

Purpose

expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL

Depositor

Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

OCRL (OCRL Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

aa 234_539

Promoter

CMV

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTU1-B-SP44s-GUS

Plasmid

#172430

Purpose

Expresses Streptomyces codon-optimised beta-D-glucuronidase

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

beta-D-glucuronidase

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

SP44s

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald-PDHA1-N-10

Plasmid

#54225

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

PDHA1 (PDHA1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
NG-ABE8-dimer

Plasmid

#138497

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Kozak-rhTRIM5alpha-PFOS

Plasmid

#79038

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

rhesus TRIM5alha-PFOS

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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mCit(P5f-P13f)3-gs

Plasmid

#228080

Purpose

Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, P5f, P13f

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(P6f)3(P14f)3-gs

Plasmid

#228081

Purpose

Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, P6f, P14f

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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ECFP-IKB

Plasmid

#111193

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Tags

ECFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-cTF

Plasmid

#87743

Purpose

IPTG-inducible expression of ligase-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and cTF (chimeric transcription factor base…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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NLS pEGFP-N3 (1299)

Plasmid

#62043

Purpose

mammalian expression of NLS-EGFP

Depositor

Eric Schirmer

Article

Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

Insert

SV40 NLS

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pCDHblast-flx-sORF-Katushka

Plasmid

#179457

Purpose

Lentiviral vector encoding a CRE inducible Katushka gene

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

loxP-shuffledORF-loxP-Katushka

Use

Lentiviral

Promoter

CMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223-BILF2

Plasmid

#195788

Purpose

Gateway Entry Clone of Epstein-Barr virus BILF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BILF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2)3(S4)3-SAH

Plasmid

#228069

Purpose

Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with long rigid linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs_NMNAT3-FLAG

Plasmid

#133261

Purpose

Express FLAG-tagged NMNAT3 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT3 (NMNAT3 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDS2142

Plasmid

#184273

Purpose

Tet-off genome insertion system

Depositor

Dominique Sanglard

Article

Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

Insert

CatTA

Expression

Yeast

Promoter

TDH3

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2)2(S4)2-gs

Plasmid

#228072

Purpose

Mammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-Dendra

Plasmid

#140559

Purpose

Red to green photoconvertible chromosome marker

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

H2B

Tags

Dendra

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-PCBP1

Plasmid

#16177

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Poly(rC) binding protein 1 (PCBP1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only