Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,415 results for: PEP;

Showing: 5241 - 5260 of 5415 results

  2. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

    Enlarge

  3. CXCR4-DuET

    Plasmid
    #213221
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213221-plasmid-map-sequence-id-430783

    Enlarge

  4. CCR6-DuET

    Plasmid
    #213202
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR6 (CCR6 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213202-plasmid-map-sequence-id-430765

    Enlarge

  5. Ad:2CMV_A2R2-N25Ctr

    Plasmid
    #122243
    Purpose
    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Article
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…
    Promoter
    CMV
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122243-plasmid-map-sequence-id-239188

    Enlarge

  7. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid
    #109426
    Purpose
    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109426-plasmid-map-sequence-id-212924

    Enlarge

  10. AP-APP

    Plasmid
    #196706
    Purpose
    Expresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cells
    Depositor
    Martino Calamai
    Article
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Use
    Tags
    AP, Avitag and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196706-plasmid-map-sequence-id-386447

    Enlarge

  13. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

    Enlarge

  15. pCEP4-PDGFRA-reporter

    Plasmid
    #136456
    Purpose
    Mammalian fluorescent reporter plasmid for PDGFRA signaling.
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Inserts
    PDGFRalpha (PDGFRA Human)
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
    Use
    Tags
    Extracellular c-myc epitope tag, Signal peptide f…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and Minimal promoter with artificial SRE enha…
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136456-plasmid-map-sequence-id-263271

    Enlarge

  18. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Use
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135014-plasmid-map-sequence-id-267599

    Enlarge

  20. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Use
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135013-plasmid-map-sequence-id-267601

    Enlarge

Showing: 5241 - 5260 of 5415 results