59,541 results

1. NeonGreen-C1-ORP3

   Plasmid

   #220070

   Purpose

   overexpresses ORP3 with a neonGreen Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL3 (OSBPL3 Human)
   

   Expression

   Mammalian

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Frt-hspB3

   Plasmid

   #63094

   Purpose

   Expression of HSPB3 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb3 (HSPB3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-NR4A1

   Plasmid

   #192925

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR4A1 (NR4A1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Solo-mScarlet-TUBA1B

   Plasmid

   #207764

   Purpose

   Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDONR221_SLC25A25

   Plasmid

   #131992

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A25 (SLC25A25 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PGKp-GFP-TAZ

   Plasmid

   #174176

   Purpose

   Lentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMaCTag-P19

   Plasmid

   #124789

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry-mNeonGreen
   

   Use

   Pcr template

   Tags

   mCherry-mNeonGreen

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDUAL CD81 (GFP)

   Plasmid

   #86980

   Purpose

   Lentiviral expression construct encoding CD81 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   CD81 (CD81 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV/hPGK

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDESTmycDICER

   Plasmid

   #19873

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   dicer 1, ribonuclease type III (DICER1 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. Halo-Sec31

    Plasmid

    #166895

    Purpose

    mammalian expression of Sec31 tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    Sec31
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-BLM

    Plasmid

    #110299

    Purpose

    Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase

    Depositor

    Chris Kok-Lung Chan

    Insert

    BLM (Bloom's Syndrome) helicase (BLM Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATGL*-V5-APEX2

    Plasmid

    #170574

    Purpose

    A C-terminal APEX2 fusion to the human ATGL S47A protein

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    ATGL* (PNPLA2 Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    changed serine 47 to alanine

    Promoter

    CMV

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe EGFR Del1

    Plasmid

    #32062

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Del1(E746-A750)

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEmerald-CD9-10

    Plasmid

    #54029

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3-GFP-MT2A

    Plasmid

    #11613

    Depositor

    Steven Johnson

    Insert

    metallothionein 2A (MT2A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC1-V16-hGDNF

    Plasmid

    #122035

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    hGDNF (GDNF Human)
    

    Use

    AAV

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPRv2 NT sgRNA3

    Plasmid

    #164929

    Purpose

    Expresses Non-targeting sgRNA3 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA3 (verified for knockout)
    

    Use

    Lentiviral

    Promoter

    EFS promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-3c-rhVTN-N

    Plasmid

    #30225

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin with N terminus cleavage (VTN Human)
    

    Expression

    Bacterial

    Mutation

    deleted amino acids: 20*-62

    Available Since

    May 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Centrin2-N-10

    Plasmid

    #55018

    Purpose

    Localization: Centrosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Centrin2 (CETN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA Sec13 pRK5

    Plasmid

    #46332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Sec13 (SEC13 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge