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pET28a-2xMreBN-mCherry-His

Plasmid

#133626

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

2xMreB-mCherry-His

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Vti1b

Plasmid

#45914

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle transport through interaction with t-SNAREs 1B (VTI1B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pLC-ZsGreen-P2A-Hygro

Plasmid

#124301

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Zeo DEST (749-3)

Plasmid

#21562

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pH-dCas9-PBE

Plasmid

#98162

Purpose

Expresses CRISPR base editor in rice

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Rice

Expression

Plant

Promoter

Ubi

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC2

Plasmid

#183298

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-VAMP8

Plasmid

#45919

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) prME (JEV) sdm2

Plasmid

#52881

Purpose

expression of Japanese Encephalitis Virus VLPs in mammalian cells

Depositor

Yorgo Modis

Article

Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

Insert

prME (POLY Japanese encephalitis virus)

Expression

Mammalian

Mutation

sdm2

Promoter

CMV

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
p35S-A3A-PBE

Plasmid

#119773

Purpose

Conversion of C to T in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

CaMv 2x35S

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-E1347A-nCas9(D10A)-UGI

Plasmid

#187410

Purpose

DddA E1347A variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA E1347A variant

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1 WT IRES GFP

Plasmid

#133816

Purpose

Encodes full-length wild type synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Promoter

hSyn (human synapsin I promoter)

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Entry vector

Tags

His and V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pB-dCas9-PBE

Plasmid

#98159

Purpose

Expresses CRISPR base editor in maize

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Maize

Expression

Plant

Promoter

Ubi

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO V5-LUC Puro (w549-1)

Plasmid

#19785

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pL1P5 ZmUbiP:GRF-GIF:NosT

Plasmid

#198046

Purpose

MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

Depositor

Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

Insert

ZmUbiP::TaGRF4-GIF1::NosT

Use

Synthetic Biology

Promoter

Zea mays (maize) ubiquitin promoter

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-AAAAA-nCas9(D10A)-UGI

Plasmid

#187409

Purpose

DddA AAAAA variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA AAAAA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-VAMP7

Plasmid

#45920

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 7 (Vamp7 Mouse)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Promoter

GAPDH

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-V96 ELP

Plasmid

#67853

Purpose

GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

Depositor

Andrew MacKay

Article

Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

Insert

GFP fused to ELP (VPGVG) repeated 96 times

Tags

GFP fused to N-terminal of ELP

Expression

Mammalian

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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Cenp-B DBD INCENP GFP

Plasmid

#45237

Purpose

Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B-DBD, INCENP-Δcen, GFP

Tags

DNA binding domain of CENP-B and GFP

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo DEST (w18-1)

Plasmid

#17390

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi; Destination vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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A3A-Gam

Plasmid

#119769

Purpose

Conversion of C to T monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-ALFA-tag-Gal4-VP16

Plasmid

#199465

Purpose

Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

Gal4-ALFA-tag-VP16

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-H2GH

Plasmid

#182202

Purpose

Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

histone2B-HaloTag-GFP

Use

AAV

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT3B

Plasmid

#183284

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT3B

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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EYFP-GalT

Plasmid

#11936

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-N1-PHPLCd1

Plasmid

#202679

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PLCD1v2(1-170):PAmCherry1 (PLCD1 Human)

Expression

Mammalian

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PARP1

Plasmid

#183312

Purpose

All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PARP1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMK401 (Hygro-P2A-mAID-Nluc)

Plasmid

#121196

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-Nluc

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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