162,692 results

1. pMT1-24X-NOG(AtUBI10)

   Plasmid

   #202016

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Inserts

   dCas9-24XGP41
   NbGP41P-sfGFP-VP64-GB1
   

   Use

   Tags

   GB1 and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP249 pGK-dCas9-DNMT3a V3

   Plasmid

   #100938

   Purpose

   dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSN840

   Plasmid

   #184832

   Purpose

   Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

   Depositor

   Kyoko Chiba

   Article

   Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

   Inserts

   KIF5A (∆exon27) (KIF5A Human)
   KLC1 (KLC1 Human)
   

   Use

   Cre/Lox

   Tags

   His-FLAG and mScarlet-2xStrepII

   Expression

   Insect

   Mutation

   ∆exon27 form of human KIF5A

   Promoter

   p10 and polyhedrin

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRAN473

   Plasmid

   #120813

   Purpose

   E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

   Depositor

   David S. Weiss, Craig Ellermeier

   Article

   Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

   Insert

   red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. DDX21

   Plasmid

   #155510

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   T232A

   Promoter

   EF-1a

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. enOgeuIscB with ωRNA-v2

   Plasmid

   #220939

   Purpose

   CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Inserts

   CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
   OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2963

   Plasmid

   #144439

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR2C2 (NR2C2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBL090J

   Plasmid

   #195045

   Purpose

   cCadC-CCB3.5 under the control of PBAD

   Depositor

   Tina Wang

   Article

   Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

   Insert

   cCadC-CCB3.5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PEP108E

   Plasmid

   #97394

   Purpose

   binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   sfGFP1-10OPT
   

   Use

   Tags

   Plasma membrane target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid

    #132524

    Purpose

    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNC2H1 (DYNC2H1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker-TEV, Precission cleavag…

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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