We narrowed to 9,203 results for: sacs

1. pEN_TT 3xFLAG-MAVS-Ala271

   Plasmid

   #158631

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   Q271A please see depositor comments below

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLIK 3xFLAG-MAVS-Ala271 hygro

   Plasmid

   #158637

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Q271A

   Promoter

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro

   Plasmid

   #158638

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   E93Q/Q271A

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. S1 1029 CP APOBEC3 no UGI

   Plasmid

   #135352

   Purpose

   Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   rAPOBEC3-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. S1 1029 CP ABEMax strategy 2

   Plasmid

   #135358

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   WT Tada-nSpyCas9 aa 1029-evolved Tada
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEN_TT 3xFLAG-MAVS-Gln93,Ala271

   Plasmid

   #158632

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   E93Q/Q271A

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEN_TT 3xFLAG-MAVS-Ala148,Ala271

   Plasmid

   #158633

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A/Q271A

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

   Plasmid

   #158639

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A/Q271A

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLIK 3xFLAG-MAVS-Ala148 hygro

   Plasmid

   #158636

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK 3xFLAG-MAVS-Gln93 hygro

    Plasmid

    #158635

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    E93Q

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR_P4-P1R:PER36modp

    Plasmid

    #128431

    Purpose

    Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Insert

    PEROXISADE36 (AT3G50990 Mustard Weed)
    

    Use

    Gateway promoter entry clone

    Tags

    Expression

    Mutation

    Premature stop codons introduced into the Xero1 (…

    Promoter

    PEROXISADE36

    Available since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pJC20cdc8.A18T

    Plasmid

    #99360

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJC20cdc8.E31K

    Plasmid

    #99361

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 31 to Lysine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJC20cdc8.110

    Plasmid

    #99363

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine and Glutamic acid 31 to L…

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

    Plasmid

    #60394

    Purpose

    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Synthetic, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4), amino a…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. GSO2/EDA23 X079_pECIA2

    Plasmid

    #115111

    Purpose

    Bait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G44700 (GSO2 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEW108

    Plasmid

    #232823

    Purpose

    Expresses yeast compass complex in insect cells

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    

    Use

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    Set1(762-1080)

    Promoter

    polyhedrin promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLViN-E-cadherin-GFP

    Plasmid

    #229710

    Purpose

    Lentiviral expression of E-cadherin-GFPTG in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CDH1 E-CADHERIN Human (CDH1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Promoter

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIGAhRC

    Plasmid

    #112510

    Purpose

    human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

    Depositor

    Charles Miller

    Article

    Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

    Insert

    HIF1B, AhR (ARNT, AHR Human, Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1,10

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTol2-HuC(elavl3)-CaMPARI2

    Plasmid

    #137185

    Purpose

    pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
    

    Use

    Tol2 plasmid for zebrafish

    Tags

    FLAG, HA, myc

    Expression

    Mutation

    Promoter

    HuC(elavl3)

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-hATG7CS

    Plasmid

    #87868

    Purpose

    Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

    Depositor

    Isei Tanida, Eiki Kominami

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 C572S mutant (ATG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C572S mutant

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCG_SARS-CoV2 M C-V5-tag

    Plasmid

    #179978

    Purpose

    Mammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 M (M Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCG_SARS-CoV1 C-V5-tag ORF3a

    Plasmid

    #179969

    Purpose

    Mammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 ORF3a (ORF3a Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEN6-Ubc5 Sc

    Plasmid

    #83234

    Purpose

    pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Inserts

    Ubiqutin (UBC Human)
    Ubc 5 (UBC5 Budding Yeast)
    Uba 1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

    Plasmid

    #65458

    Purpose

    Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBFC0619

    Plasmid

    #186690

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCG_SARS-CoV2 N C-V5-tag

    Plasmid

    #179979

    Purpose

    Mammalian expression vector for SARS-CoV-2 N, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 N (N Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCG_SARS-CoV2 ORF8 C-V5-tag

    Plasmid

    #179990

    Purpose

    Mammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 ORF8 (ORF8 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge