We narrowed to 9,939 results for: Uty

1. pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

   Plasmid

   #105035

   Purpose

   Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

   Plasmid

   #105017

   Purpose

   Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Kcmf1 (Kcmf1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUSEss-CHIg-mG3_CH2charge

   Plasmid

   #105858

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG3 heavy chain (Ighg3 Mouse)
   

   Expression

   Mammalian

   Mutation

   mutated heavy chain of mouse IgG3, muatations: Hi…

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-shGBP1.2.mKO2

   Plasmid

   #85210

   Purpose

   TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GBP1 (guanylate binding protein 1) (GBP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCh-1522

   Plasmid

   #82428

   Purpose

   Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   nvGW182 CED 11W11A
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   amino acids 1-1158 of nvGW182 were deleted and Tr…

   Promoter

   EF1A

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCh-1526

   Plasmid

   #82429

   Purpose

   Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   nvGW182 CED 11W11A
   

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   amino acids 1-1158 of nvGW182 were deleted and Tr…

   Promoter

   CMV

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mOC-STAMP (N162D)_3xFlag-Myc-CMV26

   Plasmid

   #73578

   Purpose

   Express mouse OC-STAMP ((N162D) in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

   Insert

   OC-STAMP (Ocstamp Mouse)
   

   Tags

   3x Flag and c-Myc tag

   Expression

   Mammalian

   Mutation

   changed asparagin (N) 162 to Aspartic Acid (D)

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pmKikGR Actin

   Plasmid

   #105315

   Purpose

   Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

   Depositor

   Kenneth Yamada

   Article

   Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

   Insert

   actin beta (ACTB Human)
   

   Tags

   KikGR

   Expression

   Mammalian

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. SB-CAG-mCherry-P2A-EIF3D-IP

   Plasmid

   #236769

   Purpose

   A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D-IRES-Pac.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   EIF3D (EIF3D Human)
   

   Use

   Sleeping beauty

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP

    Plasmid

    #203965

    Purpose

    Dox inducible FLAG tag ARID1a in a sleeping beauty transposon construct

    Depositor

    Elaine Fuchs

    Article

    Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

    Insert

    ARID1A (Arid1a Mouse)
    

    Use

    Sleeping beauty transposon

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207860

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid

    #234953

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORTMAGE311B

    Plasmid

    #120418

    Purpose

    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

    Depositor

    Csaba Pál

    Article

    Szili et al bioRxiv (2018)

    Inserts

    MutL E32K
    Lambda Beta
    XylS
    

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Pm

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Human, Synthetic)
    

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSEss-CHIg-mG3_M18

    Plasmid

    #82356

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
    

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF.STAT3C.Ubc.GFP

    Plasmid

    #24983

    Purpose

    3rd generation lentiviral expression of constitutively active Stat3

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A662C, N664C (constitutively active)

    Available Since

    May 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE6a

    Plasmid

    #207851

    Purpose

    Mammalian expression of PE6a prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6a
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid

    #223509

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Expression

    Mammalian

    Promoter

    CMV, SP6 and U6

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP

    Plasmid

    #209194

    Purpose

    Cre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R230 to C230

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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