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pCE059-SiT-Cas12a-[Activ]

Plasmid

#128136

Purpose

Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Publication

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Activ]-Triplex

Use

AAV

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-iCT

Plasmid

#129094

Purpose

KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Publication

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTOX6

Plasmid

#127451

Purpose

Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

tse2 toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-AlaRS

Plasmid

#124103

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Alanyl-tRNA ligase (alaS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-M-Flag-BAP1

Plasmid

#125840

Purpose

express wild type BAP1

Depositor

Boyi Gan

Publication

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET15b-His-RF2

Plasmid

#124130

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 2 (prfB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn1-mNeonGreen

Plasmid

#99135

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHAGE-ESR1

Plasmid

#116737

Purpose

Lentiviral expression of ESR1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-FGFR1

Plasmid

#116740

Purpose

Lentiviral expression of FGFR1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pPUR-mU6-sgRNA-Sirius-8XPP7

Plasmid

#121943

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

mouse U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-HDR-mEGFP-Actin

Plasmid

#119870

Purpose

AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

Depositor

Ryohei Yasuda

Publication

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

beta Actin (Actb Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRS306-URA3-PHIS3-AqLumSynth –Sapphire

Plasmid

#116931

Purpose

Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

lumazine synthase

Tags

Sapphire

Expression

Yeast

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC1802_pmax-RBFOX1N-dCasRx-C

Plasmid

#118635

Purpose

Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RBFOX1N-dCasRx-C

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Fibronectin-human-plasma in pMAX

Plasmid

#120401

Purpose

enables eukaryotic expression of human plasma fibronectin

Depositor

Inaam Nakchbandi

Publication

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Publication

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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NES-mCherry-cPHx3

Plasmid

#116862

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Publication

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CaMKIIa-mCherry

Plasmid

#114469

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

mCherry

Use

AAV

Promoter

CaMKIIa

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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pcDNA3.1 NL

Plasmid

#113442

Purpose

NanoLuc control expression vector

Depositor

Erich Wanker

Publication

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NanoLuc

Use

Luciferase

Tags

cmyc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDXinit

Plasmid

#110101

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

Depositor

Markus Seeger

Publication

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEMHE-mEGFP-mTrim21

Plasmid

#105519

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-hnRNPU-V5

Plasmid

#35974

Depositor

Susana Valente

Publication

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)

Tags

V5

Expression

Mammalian

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHR-HNRNPA1C-mCh-Cry2WT

Plasmid

#101226

Purpose

hnRNPA1(186-320) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

hnRNPA1(186-320) (HNRNPA1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Publication

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Tags

Flag-Flag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMH-SFB-DYRK1A

Plasmid

#101770

Purpose

Mammalian expression of DYRK1A fusion protein

Depositor

Michael Huen

Publication

Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

Insert

DYRK1A (DYRK1A Human)

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP Sec22b

Plasmid

#101918

Purpose

Expresses Sec22b in mammalian cells

Depositor

Thierry Galli

Publication

Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec22b (Sec22b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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IGF1R_pLX307

Plasmid

#98344

Purpose

Lentiviral expression of IGF1R

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IGF1R (IGF1R Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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RSV-Flag-Brd4

Plasmid

#86616

Purpose

Flag tagged mouse Brd4 expression construct for cell transfection

Depositor

Mario García-Domínguez

Publication

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd4 (Brd4 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-IDH2-WT

Plasmid

#87926

Purpose

Expresses IDH2 (wild-type) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Publication

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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