We narrowed to 30,686 results for: PLE

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AlphaE

Plasmid

#124527

Purpose

pColE1_AlphaE

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pPepck1 eGFP SV40

Plasmid

#132644

Purpose

Expresses eGFP mRNA from the Drosophila Pepck1 promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

Pepck1

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-rsAKARev

Plasmid

#166231

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker (rsAKARev) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

Plasmid

#87586

Purpose

PV7 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-yibDp-matB

Plasmid

#134597

Purpose

Low Phosphate inducible matB (malonyl-CoA synthetase)

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

Malonyl CoA synthetase

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2

Plasmid

#79256

Purpose

Expresses EGFP-tagged DmPAN2 in S2 cells

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

DmPAN2 (PAN2 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAM4645

Plasmid

#85124

Purpose

NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiBC-KaiBC

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate3

Plasmid

#25942

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMBD-Gate3

Plasmid

#25959

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

GAL4 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pCH043

Plasmid

#47949

Purpose

rtTA under the control of GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCH044

Plasmid

#47950

Purpose

rtTA under the control of GAL2 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal2

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSL1103

Plasmid

#129526

Purpose

pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR15aBul

Plasmid

#21117

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-VHL

Plasmid

#29498

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-Flag-mPar6aK19A

Plasmid

#24598

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19A

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pLM032

Plasmid

#134244

Purpose

Retroviral plasmid with H3.1A EGFP insert

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human H3.1A + eGFP/HALO retroviral tagging

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pIS1-mutant Fzd3 3'UTR

Plasmid

#25023

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant Fzd3 3'UTR

Use

Luciferase

Expression

Mammalian

Mutation

miR-31 binding site in 3’ UTR mutagenized from TC…

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 34C

Plasmid

#17522

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-33

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Hy_pMtnA nLuc SV40

Plasmid

#132654

Purpose

Expresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry

Plasmid

#89057

Purpose

PV4 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

GFP-T2A-mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Crkl SH2

Plasmid

#36402

Depositor

Nora Heisterkamp

Article

de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-7

Insert

Crkl SH2 domain (CRKL Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pSL1315

Plasmid

#129527

Purpose

pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

Plasmid

#87574

Purpose

PV1 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pT7ddx19gRNA

Plasmid

#47932

Purpose

in vitro transcription of ddx19 gRNA

Depositor

Wenbiao Chen, Susan Wente

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

ddx19 gRNA (ddx19 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pQCXIN X2/pTER shLUC (w347-1)

Plasmid

#17489

Purpose

Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

RNAi and Retroviral

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-126 site

Plasmid

#25026

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-126 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A[T22N]

Plasmid

#24906

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only