159,258 results

1. pAK_DR30_CasRx_GFP

   Plasmid

   #134843

   Purpose

   All in one overexpression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   T2A EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx4-Cdc42-2G

   Plasmid

   #68814

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-HP1gamma

   Plasmid

   #17650

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 gamma (CBX3 Human)
   

   Use

   Tags

   GFP and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMV306hsp

   Plasmid

   #26155

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   hsp60 promoter
   

   Use

   Mycobacteria integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hPDGFRA-GFP

   Plasmid

   #22919

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. MZB209

   Plasmid

   #228492

   Purpose

   BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

   Depositor

   Richard Baker

   Insert

   BirA (birA )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214996

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (TMEM115 Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330-mcherry

   Plasmid

   #98750

   Purpose

   Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

   Depositor

   Jinsong Li

   Article

   Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP GR

    Plasmid

    #47504

    Purpose

    Expresses glucocorticoid receptor in mammalian cells

    Depositor

    Alice Wong

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-HcKCR1-EYFP

    Plasmid

    #182021

    Purpose

    Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

    Depositor

    Mingshan Xue

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_BiSSTe10_ChR2_mCherry

    Plasmid

    #213815

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG

    Plasmid

    #169416

    Purpose

    Used for guide cloning

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT-puro

    Plasmid

    #17923

    Purpose

    Insect expression vector

    Depositor

    David Sabatini

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Drosophila metallothionein gene promoter drives e…

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiGuide-tagBFP

    Plasmid

    #217007

    Purpose

    lentiviral plasmid for gRNA cloning with selectable tagBFP expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

    Plasmid

    #223273

    Purpose

    Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid

    #172507

    Purpose

    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pCMV - huDisCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYS16

    Plasmid

    #202082

    Purpose

    Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT mNG-T2A-PuroR Minicircle Parent
    

    Use

    CRISPR

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge