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pcDNA3-Lyn-dInPAkt(R23/25A)

Plasmid

#181835

Purpose

Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Arginines 23 and 25 in PH domain mutated to alani…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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M3-PSCFP2

Plasmid

#114185

Purpose

studying clustering of M3 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

Insert

Muscarin acetylcholine receptor 3 (CHRM3 Human)

Tags

3xHA and PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-4A-VC

Plasmid

#110649

Purpose

expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

HA and VC

Expression

Mammalian

Mutation

C379A, C385A, C421A and C427A

Promoter

CMV

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRL-TK 3'FBXW7 UTR

Plasmid

#26649

Depositor

Alex Minella

Article

Xu et al J Biol Chem. 2010 Nov 5;285(45):34439-46

Insert

FBXW7 (FBXW7 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains only the 3'UTR of FBXW7, nucleotide…

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-FLAG-NEMO-C54/347A

Plasmid

#27268

Depositor

Thomas Gilmore

Article

Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

Insert

NEMO (IKBKG Human)

Tags

FLAG

Expression

Mammalian

Mutation

Cys54Ala Cys347Ala

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pHS2056

Plasmid

#239837

Purpose

OMEGAOff + omegaRNA scaffold AAV expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold

Use

AAV

Tags

HA and SV40 NLS

Mutation

Insertion of REC domain from NbaCas9-1 + E137K/E4…

Promoter

EFS; human U6

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR(K30/32L)

Plasmid

#181833

Purpose

Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dPlcR (K30L,K32L)

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pT7NT*-Bri2 113-231 R221E

Plasmid

#138134

Purpose

Expresseds human Bri2 BRICHOS R221E mutant in E. coli

Depositor

Jan Johansson

Article

Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

Insert

human Bri2 BRICHOS R221E (ITM2B Human)

Tags

NT* tag derived from spider silk proteins

Expression

Bacterial

Promoter

T7

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKSJ376

Plasmid

#103065

Purpose

Expresses colicin E1 with R domain deletion and ImmE1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Inserts

colicin E1 immunity protein (pColE1_26, imm E. coli)
cea/colicin E1

Expression

Bacterial

Mutation

R (receptor-binding domain) deletion in colicin E…

Promoter

T7 lac (from pET) and T7 lac (from pET52-b)

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75240

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75241

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

Plasmid

#42651

Purpose

destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

Depositor

Scott Friedman

Article

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 399 SNP (TLR4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T399I

Promoter

CMV/TO

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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MTRAP-bio

Plasmid

#47746

Purpose

Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MTRAP

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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CK2alpha V66A/I174A-HA (pJD23)

Plasmid

#37362

Depositor

David Litchfield

Article

Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

Insert

CK2α V66A/I174A (CSNK2A1 Human)

Tags

HA X3

Expression

Mammalian

Mutation

V66A, I174A

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TK1

Plasmid

#20652

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TK1 (TK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-all

Plasmid

#21653

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated let-7 and miR103/107 microRNA binding sit…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pAJ20

Plasmid

#14919

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

Strongyloides stercoralis Ribosomal protein small subunit

Tags

GFP(S65C)

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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