We narrowed to 63,680 results for: cloning
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Plasmid#121119PurposeEndogenous gene activation with the SunTag CRISPR activation systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-GCN4x10_scFv-VP64
UseCRISPRExpressionMammalianPromoterTet-ONAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ArchT-BFP2-TSERex
Plasmid#123312PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArchT-BFP2
ExpressionMammalianPromoterCAGAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianPromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-T2A-HygR
Plasmid#118153PurposeSpCas9 expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9-T2A-HygR
UseCRISPRTags3XFLAGExpressionMammalianPromoterCAG and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAR18_StrepI_mFASm_H8_pET22b
Plasmid#122847PurposeExpresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertmouse FASN/ mouse fatty acid synthase (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-LACTB-dAPEX2
Plasmid#117179PurposePlasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAVExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EPHA2
Plasmid#116732PurposeLentiviral expression of EPHA2DepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/Envelope
Plasmid#120821PurposeEctopic expression of ZIKV proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEnvelope ZIKV
TagsV5ExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
TagsSapphireExpressionYeastPromoterINO4Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MiniCoopR mitfa:EGFP
Plasmid#118850PurposeExpresses EGFP and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertmitfa: EGFP (mitfa Zebrafish, Synthetic)
UseZebrafish expression, tol6Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79
Plasmid#111729PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ79 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE142
Plasmid#118494Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
ExpressionBacterialAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH533_MBP-Cas14b2 expression
Plasmid#112506PurposeExpresses 10xHis-MBP fused to Cas14b2 under t7 promoterDepositorInsertCas14b2
UseCRISPRTagsMBPExpressionBacterialPromoterT7Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR1307
Plasmid#111133PurposeExpresses WDR48.DepositorAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-RFP-HYGRO
Plasmid#115145PurposeSafe harbor site 231 knock-in vector with RFP-HygromycinR expression cassetteDepositorInsertRFP
ExpressionMammalianPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion mAID-mCherry with Neo
Plasmid#113831PurposeEasyFusion vector for targeting endogeneous genes with mAID-mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAID-mCherry
UseSynthetic BiologyAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS9
Plasmid#111762PurposeExpresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-GGSGGS9-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR1-optoDroplet
Plasmid#111509PurposeFor optogenetic clustering of FGFR1DepositorInsertFGFR1 (FGFR1 Human)
UseLentiviralTagsCry2, FUS, Fusion Red, and Myristoylation sequenc…PromoterSFFVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Myristoylated optoDroplet
Plasmid#111507PurposeFor membrane localized optoDropletsDepositorInsertCry2 (CRY2 Mustard Weed)
UseLentiviralTagsFUS, Fusion Red, and Myristoylation Sequence (MYR…PromoterSFFVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-EFS-HypaSpCas9-P2A-EGFP
Plasmid#106964Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralExpressionMammalianPromoterEFSAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only