We narrowed to 6,126 results for: tTA

Showing: 5341 - 5360 of 6126 results

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pLenti-Blast-sgAMPKa1

Plasmid

#138704

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1-mCherry-Puro

Plasmid

#193763

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1-mCherry (CDT1 Human)

Use

Lentiviral

Tags

WT

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔPIP-mCherry-Puro

Plasmid

#193765

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔPIP-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

PIP degron removed (a.a.1-19 removed)

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PIK3C2A gRNA (BRDN0001149181)

Plasmid

#76414

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2A (PIK3C2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX335-NQL003-WAPL-sgRNA2

Plasmid

#175551

Purpose

For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nickase and sgRNA against mouse WAPL STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-NDP52

Plasmid

#187828

Purpose

Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

GST-TEV

Expression

Bacterial

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-ARshRNA2

Plasmid

#220562

Purpose

To inducibly knockdown AR expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

AR shRNA2 (AR Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgAMPKa1

Plasmid

#138684

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro hGSDME gRNA1

Plasmid

#223519

Purpose

knocking out hGSDME in human cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PEX3

Plasmid

#227303

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PEX3 (PEX3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-LRPPRC-ts1

Plasmid

#174268

Purpose

LRPPRC knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

LRPPRC shRNA (LRPPRC Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgVRK1

Plasmid

#199638

Purpose

Tamoxifen-inducible expression of sgRNA targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔCy-mCherry-Puro

Plasmid

#193764

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔCy-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TBK1 gRNA (BRDN0001145663)

Plasmid

#76361

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBK1 (TBK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.5 shETV4-B

Plasmid

#74976

Purpose

shETV4 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 Human)

Use

RNAi

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

Plasmid

#192682

Purpose

Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-EIF2AK2-ts1

Plasmid

#174247

Purpose

EIF2AK2 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

EIF2AK2 shRNA (EIF2AK2 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLC-CUL4A K705R-P2A-Puro

Plasmid

#124305

Purpose

Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Lysine 705 to Arginine

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shOCT4

Plasmid

#198761

Purpose

conditional knockdown of OCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shOCT4 (POU5F1 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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