We narrowed to 5,867 results for: nls

1. 766 p3E-2A-nlsmCherrypA

   Plasmid

   #195972

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-nlsmCherry (nuclear)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN017-SpCas9-hiNLS-t-M4

   Plasmid

   #234455

   Purpose

   Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-TagRFP-NLS

   Plasmid

   #184446

   Purpose

   Nucleus-targeted TagRFP expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   TagRFP-3xNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA_Lifeact-GFP_NLS-mCherry

   Plasmid

   #69058

   Purpose

   Dual Fluorescent Reporter for F-actin and Nuclei

   Depositor

   Olivier Pertz

   Insert

   LifeAct-GFP_IRES_NLS-mCherry
   

   Use

   Tags

   Lifeact-GFP, NLS-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-LgBiT-FRB

   Plasmid

   #183403

   Purpose

   Expresses nuclear-localized LgBiT-FRB

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-FRB
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-GT_CAG_3xTurboGFP-NLS

   Plasmid

   #229777

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   3x TurboGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. epB_CAG_TetRFlag-nls-GFPx2_DEx2

   Plasmid

   #119910

   Purpose

   ePiggyBac mammalian expression plasmid for TetR-GFP fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   TetRFlag-nls-GFPx2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-NLS-actin

   Plasmid

   #118380

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   NLS

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-AKAR4-NLS

   Plasmid

   #138217

   Purpose

   Nuclear-targeted FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   AKAR4-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMHE-NLS-mEGFP

    Plasmid

    #105527

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Nucleoplasmin
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    NLS of nucleoplasmin (encoding amino acids KRPAAT…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTetOne-tdTomato-cNLS

    Plasmid

    #138529

    Purpose

    Inducible expression of tdTomato with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Use

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3Gs

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNLS-LEXY (pDN138)

    Plasmid

    #72657

    Purpose

    golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Tags

    NLS and mCherry-LEXY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-CMV-nlsCRE

    Plasmid

    #12265

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLew100::NLS-ISceI-HA

    Plasmid

    #21299

    Depositor

    Nina Papavasiliou

    Article

    Boothroyd et al Nature. 2009 Apr 15. ():.

    Insert

    I-SceI (SCEI )
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-BiNLS-fYap

    Plasmid

    #176623

    Purpose

    expresses optofYap in zebrafish

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    Yes associated protein (yap1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQC NLS-CFP IX

    Plasmid

    #37334

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    NLS CFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF1a-hMLH1NTD-NLS

    Plasmid

    #174826

    Purpose

    Mammalian expression of human MLH1NTD-NLS (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1_NTD-NLS (codon optimized)
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    truncation of residues 1-335

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSyn1-2xNLS-mTurquoise2

    Plasmid

    #118025

    Purpose

    An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-mTurquoise2
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBbsr2-EKARrEV-NLS

    Plasmid

    #173855

    Purpose

    Encoding EKARrEV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

    Plasmid

    #32627

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Donor + Sce BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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