Addgene

We narrowed to 22,147 results for: Sele

Showing: 781 - 810 of 22147 results

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pDual_dsCas9_BFP_hs_NC_chr1

Plasmid

#214686

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

Depositor

Kevin Janes

Insert

dgRNA_Chr1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDual_dsCas9_BFP_hs_TP73

Plasmid

#214687

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73

Depositor

Kevin Janes

Insert

dgRNA_TP73 (TP73 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNX3s2-PK3

Plasmid

#78394

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
zeoCV

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3s2-FL3

Plasmid

#78395

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
zeoCV

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJQ200mp19

Plasmid

#78501

Purpose

Suicide vector for allelic exchange

Depositor

Michael Hynes

Article

Quandt et al Gene. 1993 May 15;127(1):15-21.

Type

Empty backbone

Use

Suicide vector for gene replacement

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
pSA40

Plasmid

#35114

Depositor

John McCusker

Article

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

lys5 flanked by loxp

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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FC511

Plasmid

#27330

Depositor

Fred Cross

Article

Cross et al Yeast. 2010 Oct 22. ():.

Insert

CDC28 (CDC28 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

mutation CDC28-1N (P250L)

Promoter

Available since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
FC513

Plasmid

#27332

Depositor

Fred Cross

Article

Cross et al Yeast. 2010 Oct 22. ():.

Insert

CDC28 (CDC28 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut A

Plasmid

#11314

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation A (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut B

Plasmid

#11315

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation B (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut C

Plasmid

#11316

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation C (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut D

Plasmid

#11317

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation D (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut E

Plasmid

#11318

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation E (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut F

Plasmid

#11319

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation F (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut H

Plasmid

#11321

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation H (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut GFP

Plasmid

#11323

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pTNS3

Plasmid

#63127

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

P1 and Ptac

Available since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+8CeGFP

Plasmid

#34952

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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HO-Poly-KanMX4-HO

Plasmid

#51662

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSPble

Plasmid

#63561

Purpose

Bleomycin drug selection marker

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Bleomycin drug selection marker

Use

Leishmania expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pTW358f

Plasmid

#227935

Purpose

Express hIAPP-T7n

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

hIAPP-T7n

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pbacPuroR

Plasmid

#26348

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Insert

Bacterial promoter::PuroR::bacterial 3p flank

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pNIC-GST-TEV-GG

Plasmid

#215795

Purpose

Cloning of target gene with N terminal GST tag and TEV cleavage

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNIC-CT-sfGFP-HN-GG

Plasmid

#215803

Purpose

Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLOVE empty vector

Plasmid

#15948

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCherry

Plasmid

#34935

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pNIC-CTH10STII-GG

Plasmid

#215804

Purpose

Cloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tag

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNX3b2-FL3

Plasmid

#78388

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
hygML

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNXRVb-HACre

Plasmid

#78401

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
hygMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2+8NeGFP

Plasmid

#34953

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge