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Showing: 1041 - 1080 of 11031 results

  8. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid
    #172317
    Purpose
    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    172317-plasmid-map-sequence-id-338787

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  12. MuHKI-pGFPN3

    Plasmid
    #21919
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the full length human HKI cDNA sequence w…
    Promoter
    Available since
    Nov. 2, 2009
    Availability
    Academic Institutions and Nonprofits only

  14. ATG-1929

    Plasmid
    #108712
    Purpose
    CBR2opt in pF4Ag expression vector with T7 and CMV promoter
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    T7, CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108712-plasmid-map-sequence-id-224541

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  16. CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

    Plasmid
    #117384
    Purpose
    An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Insert
    GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117384-plasmid-map-sequence-id-225349

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  24. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid
    #58490
    Purpose
    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Glutamine
    Promoter
    Ubiquitin
    Available since
    Aug. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    58490-plasmid-map-sequence-id-90354

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  25. ATG-2460

    Plasmid
    #108713
    Purpose
    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt-copGFP fusion
    Use
    Lentiviral
    Tags
    CBR2opt fused to copGFP
    Expression
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    elongation factor-1
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108713-plasmid-map-sequence-id-224540

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  27. NST1_pOpOn2.1

    Plasmid
    #228065
    Purpose
    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Charles Anderson
    Article
    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228065-plasmid-map-sequence-id-456794

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  28. NST3_pOpOn2.1

    Plasmid
    #228066
    Purpose
    Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Charles Anderson
    Article
    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3
    Available since
    Jan. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228066-plasmid-map-sequence-id-456796

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  31. pKM 1995

    Plasmid
    #217826
    Purpose
    Expresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.
    Depositor
    Aziz Sancar
    Article
    Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.
    Insert
    HY4 (CRY1 Mustard Weed)
    Use
    Tags
    Maltose-binding protein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217826-plasmid-map-sequence-id-434880

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  35. TRE-mClover3-TDP-43ΔNLS

    Plasmid
    #214917
    Purpose
    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta
    Depositor
    Ophir Shalem
    Article
    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d
    Insert
    TDP-43ΔNLS (TARDBP Human)
    Use
    Lentiviral
    Tags
    mClover3
    Expression
    Mutation
    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214917-plasmid-map-sequence-id-424953

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  37. pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

    Plasmid
    #197599
    Purpose
    Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive
    Depositor
    Alexander Gottschalk
    Article
    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:
    Insert
    Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    Use
    Tags
    Expression
    Worm
    Mutation
    D387A, E490G
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    197599-plasmid-map-sequence-id-410713

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Showing: 1041 - 1080 of 11031 results