-
Plasmid#160777PurposeSuppress DDX11DepositorInsertshDDX11_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
HMGA2(3'UTR DEL)+GFP
Plasmid#25406DepositorInserthigh mobility group AT-Hook 2 (Hmga2 Mouse)
UseLentiviralTagsExpressionMammalianMutation3" UTR is absentPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
XE257 pCDNA3.1(zeo)-hDsh3-truncDEP
Plasmid#16752DepositorInsertFLAG-hDsh3-trunc before DEP (DVL3 Human)
UseTagsflagExpressionMammalianMutationtruncated before DEPPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-HA
Plasmid#105785PurposeExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_EE-NG-nCas9
Plasmid#163542PurposeMammalian CG-to-GC base editingDepositorInsertEE-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…PromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper shAngptl4 hygro
Plasmid#19154DepositorInsertsmall hairpin RNA against angiopoietin-like 4 (ANGPTL4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H2BC11-MMEJ
Plasmid#207760PurposeMMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-APPL1-ΔPTB
Plasmid#59768PurposeExpresses APPL1 with PTB domain deletedDepositorInsertAPPL1 (APPL1 Human)
UseTagsEGFPExpressionMammalianMutationdeleted amino acids 467-709PromoterCMVAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLE2
Plasmid#160766PurposeSuppress POLE2DepositorInsertshPOLE2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Elafin
Plasmid#18102DepositorInsertElafin (PI3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
AfrLEA3m_mCh-2xFKBP (pBS1137)
Plasmid#185321PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertAfrLEA3m
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_APOE_HUMAN_E4_47-300 (pBS0847)
Plasmid#185279PurposeFor the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAPOE_HUMAN_E4_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-YOD1-Strep-HA
Plasmid#113483PurposeExpression of human YOD1 with C-terminal strep-HA tagDepositorInsertYOD1 (YOD1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Gh
Plasmid#44507DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLD1_1
Plasmid#160791PurposeSuppress POLD1DepositorInsertshPOLD1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P REV3L_2
Plasmid#160798PurposeSuppress REV3LDepositorInsertshREV3L_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P REV3L_1
Plasmid#160797PurposeSuppress REV3LDepositorInsertshREV3L_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLA1_2
Plasmid#160790PurposeSuppress POLA1DepositorInsertshPOLA1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P POLA1_1
Plasmid#160789PurposeSuppress POLA1DepositorInsertshPOLA1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only