We narrowed to 8,526 results for: construct
-
-
pWJPrP31
Plasmid#22105DepositorInsertPrP exon 3 EcoR1 fragment
UseConstruction of targeting vectorMutationgenomic EcoR1 fragment containing exon 3 of PrPAvailable SinceOct. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWJPrP34
Plasmid#22107DepositorInsertPrP exon 3 EcoR1 fragment
UseConstruction of targeting vectorMutationAdded a Cla1 site right before translation start …Available SinceOct. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
AB65_pCAG.Cas9-D10A.rBGpA
Plasmid#199256PurposeExpression construct encoding the SpCas9-D10A nickaseDepositorInsertSpCas9.D10A nickase
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG FUCCI
Plasmid#175266PurposeBicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesisDepositorInsertFUCCI
ExpressionMammalianPromoterCAGAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetOn-mCherry-eGFP-RAMP4
Plasmid#109014PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)DepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianPromoterCMV promoterAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 mCherry-NLS
Plasmid#58476PurposeExpresses a nuclear-targeted mCherry control construct, on a CMV reporterDepositorInsert3X NLS with 3X stop codons
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX304-Flag-APEX2-NES
Plasmid#92158PurposeCytosolic APEX2 constructDepositorInsertFlag-APEX2-NES
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-alphaTubulin_C1
Plasmid#189768PurposeConstruct for labelling microtubules in mammalian cells with mScarlet3-alphatubulinDepositorInsertmScarlet3-alphaTubulin
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16
Plasmid#71728Purposeplasmid expressing fusion construct of Gal4 DBD and VP16 ADDepositorAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_25bpLinker
Plasmid#157788PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_25bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
Rad51–Cas9(D10A)
Plasmid#125567PurposeExpresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAIDA1
Plasmid#79180PurposeA construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tagsDepositorInsertAdhesin Involved in Diffuse Adherence
TagsHis and MycExpressionBacterialPromoterlacUV5Available SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH147
Plasmid#90991PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.DepositorInsertgRNA conserved, FRT, DS_HIS1
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_20bpLinker
Plasmid#157787PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_20bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-WT
Plasmid#64060PurposeEGFP fused at the N-terminus of tubulin; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_30bpLinker
Plasmid#157789PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_30bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GLUT1-GFP
Plasmid#89440PurposeGLUT1 constructDepositorAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only
