We narrowed to 15,827 results for: grna

1. pPN155

   Plasmid

   #91684

   Purpose

   Express sgRNA targeting human ZNF536

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   ZNF536 (ZNF536 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTX203

   Plasmid

   #89267

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsDEP1-gRNA02
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTX202

   Plasmid

   #89266

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsDEP1-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTX200

   Plasmid

   #89264

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsYSA-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTX199

   Plasmid

   #89263

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA02

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsPDS-gRNA02
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCas56

   Plasmid

   #82394

   Purpose

   sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas55

   Plasmid

   #82393

   Purpose

   sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6_(GLuc)_INT[S1Apt]

   Plasmid

   #68425

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct with S1 streptavidin aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196082

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Aminoglycoside phosphotransferase
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196080

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_045

    Plasmid

    #107144

    Purpose

    cloning of a sgRNA

    Depositor

    David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. L4440gtwy

    Plasmid

    #11344

    Depositor

    Guy Caldwell

    Insert

    gateway cassette
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-shControl

    Plasmid

    #242699

    Purpose

    shRNA control

    Depositor

    Xingguo Zhu

    Insert

    shControl
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT077

    Plasmid

    #137879

    Purpose

    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ141D-peg

    Plasmid

    #141081

    Purpose

    Gateway compatible prime editing gRNA empty backbone vector

    Depositor

    Yiping Qi

    Article

    Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRDA_550

    Plasmid

    #203398

    Purpose

    All-in-one lentiviral expression of gRNA and EnAsCas12a

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Insert

    Enhanced Acidaminococcus Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX459-APP-KO

    Plasmid

    #176487

    Purpose

    To knockout APP gene

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JD633

    Plasmid

    #160393

    Purpose

    CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Inserts

    ZmUbi::wheat GRF4-GIF1 chimera
    ZmUbi:TaCas9 + TaU6:gRNA
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pDIRECT_23A

    Plasmid

    #91138

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMOD_B2520

    Plasmid

    #91077

    Purpose

    Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    OsU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pB025

    Plasmid

    #183092

    Purpose

    Expresses FnCas12a in Bacteroides and used for genome editing

    Depositor

    Lei Dai

    Article

    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

    Insert

    FnCas12a, gRNA, Promoter, HAB, TetR,
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1TDPGH023

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQ132B2.0

    Plasmid

    #99888

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SiC-V1-Scr

    Plasmid

    #133042

    Purpose

    SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX330-EN1201

    Plasmid

    #92144

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge