We narrowed to 10,009 results for: ADA

1. pCMV_RBMX-eA3A-UdgX-HF-nCas9

   Plasmid

   #163548

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-HF-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

   Promoter

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

   Plasmid

   #163552

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-nCas9-RBMX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-PRAS40 WT

   Plasmid

   #86758

   Purpose

   N-terminal HA-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   human PRAS40 (AKT1S1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDicAID_nCas9-PmCDA-2A-NptII_ETR

   Plasmid

   #91695

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGME

   Plasmid

   #71252

   Purpose

   luciferase reporter with GM-CSF promoter + 717 bp Bgl II GM-CSF enhancer

   Depositor

   Peter Cockerill

   Article

   Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

   Insert

   GM-CSF enhancer (CSF2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   GM-CSF

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NSK026 CMV-TO-p53R248W (FLP-IN)

   Plasmid

   #191168

   Purpose

   Expression of human p53 mutant R248W in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   p53(R248W) (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R248W

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSW648 - pREV>>

   Plasmid

   #115996

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Use

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 MP1 shRNA

   Plasmid

   #26632

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   MP1 shRNA (LAMTOR3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-Tag2-Sox9 T236A

   Plasmid

   #111455

   Purpose

   Transient expression of Flag-tagged human Sox9 with T236A mutation

   Depositor

   Sharon Pine

   Article

   Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

   Insert

   Sox9 (SOX9 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T236A mutation

   Promoter

   CMV

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2R3a-3AT

    Plasmid

    #71273

    Purpose

    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. H2B-mAvicFP1

    Plasmid

    #129511

    Purpose

    Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    H2B-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 fused to the C terminus of human histone…

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EFS-DIO-GRAB_OTmut

    Plasmid

    #185389

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

    Plasmid

    #226350

    Purpose

    Expression of proteasome substrate with tail for molecular ruler

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5FRT/TO-Cezanne-Strep-HA

    Plasmid

    #113492

    Purpose

    Expression of human Cezanne with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Cezanne (OTUD7B Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-mCherry-C1-iChR2++

    Plasmid

    #98172

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChR2++ gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-C9ORF80

    Plasmid

    #128418

    Purpose

    Express C9ORF80 in E. coli with a His tag (C-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    C9ORF80 (INIP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. WDR5-HaloTag

    Plasmid

    #200881

    Purpose

    Mammalian expression of human WDR5 with C-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    WDR5-HaloTag (WDR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNH-TrxT-G-S18

    Plasmid

    #127827

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_SEPT9_i3-msfGFP

    Plasmid

    #180322

    Purpose

    mammalian expression of human SEPT9_i3 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_RBMX-eA3A-UdgX-nCas9

    Plasmid

    #163546

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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