159,284 results

1. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-IPP1-His

   Plasmid

   #124137

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Inorganic pyrophosphatase (IPP1 Budding Yeast)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-mVenus-CAAX

   Plasmid

   #75153

   Purpose

   Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mVenus-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PSL1180polyUBdsRED

   Plasmid

   #49327

   Purpose

   Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

   Depositor

   Leslie Vosshall

   Insert

   dsRED
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Aedes polyubiquitin

   Available since

   Nov. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1-IL6

   Plasmid

   #111933

   Purpose

   Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

   Insert

   Interleukin 6 (IL6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   CMV

   Available since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ac5-STABLE2-puro

   Plasmid

   #208032

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Use

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV_LCMV_GPC

   Plasmid

   #207288

   Purpose

   Mammalian expression of Lymphocytic choriomeningitis virus GPC protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   LCMV_GPC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Roxed-Cre

   Plasmid

   #51273

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Roxed-Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCL/IFNB1-d2eGFP-3’UTR

    Plasmid

    #180232

    Purpose

    Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

    Insert

    d2eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1 kb upstream of IFNB1 CDS

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-FOXA2

    Plasmid

    #153110

    Purpose

    Expression of Flag tagged human FOXA2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXA2 (FOXA2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLT3-MSCV-IRES-GFP

    Plasmid

    #184778

    Purpose

    Expresses wildtype FLT3 in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. (189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

    Plasmid

    #160739

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nGFP-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Ki67-mCherry

    Plasmid

    #183748

    Purpose

    Expresses EGFP-Ki67-mCherry

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNP1723

    Plasmid

    #222727

    Purpose

    sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

    Plasmid

    #216526

    Purpose

    Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-26b-NbVHH05-ALFA-His

    Plasmid

    #171569

    Purpose

    Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-ALFA-His (UBE2J1 Human)
    

    Use

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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