We narrowed to 17,206 results for: puro

1. p8325 pLIX YAP1 WT

   Plasmid

   #184527

   Purpose

   Expression of YAP1 WT

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 WT (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 and H1

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p6622 MSCV-P C HA GLTSCR

   Plasmid

   #34902

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   GLTSCR1 (BICRA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX307-VHL(C162F)-3F-BirA

   Plasmid

   #220149

   Purpose

   Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   changed cysteine 162 to phenylalanine; codon opti…

   Promoter

   EF-1a

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-mGCN2-FLAG-IP

   Plasmid

   #101794

   Purpose

   Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

   Depositor

   Seiichi Oyadomari

   Article

   Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

   Insert

   Gcn2 (Eif2ak4 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-iT7

   Plasmid

   #211699

   Purpose

   NIS-Seq-compatible lentiviral vector for expressing sgRNAs

   Depositor

   Jonathan Schmid-Burgk

   Article

   Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIP-mCherry-CDMPR

   Plasmid

   #182643

   Purpose

   Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   CDMPR
   

   Use

   Retroviral

   Tags

   N-terminal ER targeting sequence of hemagglutinin…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpRY Cas9

   Plasmid

   #188491

   Purpose

   Expresses FLAG tagged SpRY Cas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIP-EGFP-CDMPR

    Plasmid

    #182642

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    CDMPR
    

    Use

    Retroviral

    Tags

    EGFP and N-terminal ER targeting sequence of hema…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. W118-1_hTGM2-V5

    Plasmid

    #180413

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX317-EGFP

    Plasmid

    #115439

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP

    Plasmid

    #215055

    Purpose

    Expresses EGFP in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. hMLKL-Venus

    Plasmid

    #106078

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    human MLKL-Venus (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Dox-inducible

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLX_307 SLC31A1

    Plasmid

    #184491

    Purpose

    Lentivirus expression for SLC31A1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    SLC31A1 (SLC31A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRXIP TMEM41B-GFP

    Plasmid

    #117414

    Purpose

    Expresses TMEM41B tagged with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    TMEM41B (TMEM41B Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMV_AA050

    Plasmid

    #216107

    Purpose

    gDNA expression for Cas9 with ChenF+E trRNA (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gDNA expression for Cas9 with ChenF+E trRNA
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-Tet-OsTIR1(WT)-V5

    Plasmid

    #158663

    Purpose

    Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(WT) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-opto-hCaspase-8

    Plasmid

    #208786

    Purpose

    Inducibly expresses human Caspase 8 fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase 8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OMM-V5-LOV-Turbo

    Plasmid

    #199665

    Purpose

    expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    AKAP1 transmembrane domain and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLPCX mito Grx1-roGFP2

    Plasmid

    #64977

    Purpose

    Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. VCP F12.4 gRNA

    Plasmid

    #90939

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F12.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. VCP F11.4 gRNA

    Plasmid

    #90938

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F11.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBMN-HA-TBK1

    Plasmid

    #199205

    Purpose

    Used for the expression of TBK1 (SMC Internal No. 1996)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPV-EF1a-mRxxFP1(AfeI)-iP-A

    Plasmid

    #204625

    Purpose

    Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BRCA2 A12.2 gRNA

    Plasmid

    #90551

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA2 (Guide Designation A12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRXIP TMEM41B-3xFLAG

    Plasmid

    #117415

    Purpose

    Expresses TMEM41B tagged with 3xFLAG in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    TMEM41B (TMEM41B Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMXs-IP spGFP-ERGIC53

    Plasmid

    #38270

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Use

    Retroviral

    Tags

    EGFP with Calreticulin signal peptide at N-termin…

    Expression

    Mammalian

    Mutation

    deleted signal peptide (1-30 amino acids)

    Promoter

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSBbi 41BBL CD86 HLA-A201

    Plasmid

    #183248

    Purpose

    Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Inserts

    CD86, 41BBL
    HLA-A2 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRExT2A-CT-PmNG

    Plasmid

    #232201

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLVX-opto-hCaspase-4 dCARD

    Plasmid

    #208785

    Purpose

    Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry Caspase4 dCARD (CASP4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSCV-mCherry-Syk

    Plasmid

    #50045

    Purpose

    Expression of mCherry-Syk in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    Spleen tyrosine kinase (Syk) (Syk Mouse)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRetroX GFP T2A Cre

    Plasmid

    #63704

    Purpose

    Inducible expression of GFP T2A Cre fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    T2A Cre
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLCRISPR-CMV-cGAS3

    Plasmid

    #102610

    Purpose

    CRISPR/Cas9 lentivector targeting human cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    gRNA targeting human cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. shTBX5 # 2

    Plasmid

    #42550

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

38. shTBX5 # 1

    Plasmid

    #42549

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pAAVS1-PC-GCaMP6f

    Plasmid

    #73503

    Purpose

    GCaMP6f knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge