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Showing: 8281 - 8310 of 173280 results

  6. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  11. AAV-CAG-M-SPOTIT2

    Plasmid
    #191489
    Purpose
    Green fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-SPOTIT2
    Use
    AAV
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191489-plasmid-map-sequence-id-378568

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  15. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Insert
    Cas9
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Promoter
    none
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  18. pCAG2-EGFP-C-IFT140

    Plasmid
    #218739
    Purpose
    Expresses C-terminally EGFP-tagged IFT140 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT140 (IFT140 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218739-plasmid-map-sequence-id-434233

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  22. pAAV-syn-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234438
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi
    Has Service
    AAV1
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234438-plasmid-map-sequence-id-465745

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  29. pDAC446

    Plasmid
    #195239
    Purpose
    Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195239-plasmid-map-sequence-id-382947

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Showing: 8281 - 8310 of 173280 results