164,722 results

1. pEGFP-ORP8-H514A-H515A

   Plasmid

   #208360

   Purpose

   Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   ORP8 (OSBPL8 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H514A-H515A

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDisplay-GRAB_CRFmut

   Plasmid

   #208656

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3-DCX-TurboID-V5

   Plasmid

   #190740

   Purpose

   Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   doublecortin (DCX Human)
   

   Use

   Tags

   TurboID and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-sgKeap1#1/Cre

   Plasmid

   #173639

   Purpose

   Expresses a Keap1-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Keap1 (Keap1 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX2T-TEV CHMP1B 1-143

   Plasmid

   #108286

   Purpose

   Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-143

   Promoter

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pM-TagRFP657

   Plasmid

   #176563

   Purpose

   Expression of TagRFP657 for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZBTB4-donor

   Plasmid

   #176374

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZBTB4 (ZBTB4 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDM326_DPAKa(GBD)-YFP

   Plasmid

   #128701

   Purpose

   expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

   Insert

   GBD domain (aa 731-890) of DPAKa
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP

   Expression

   Mutation

   none

   Promoter

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

   Plasmid

   #111923

   Purpose

   Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_19μ
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, F92W, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CX3CR1-EGFP

    Plasmid

    #24061

    Depositor

    Dan Littman

    Insert

    CX3CR1 (Cx3cr1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.2 YFP

    Plasmid

    #84910

    Purpose

    expression of YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Brm WT-Flag pShuttle

    Plasmid

    #122105

    Purpose

    Expression of full length Brm in mammalian cells

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    BRM (SMARCA2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiCRISPR v2-sgLKB1 clone2

    Plasmid

    #162126

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT2/LTR7-GFP

    Plasmid

    #62541

    Purpose

    LTR7-driving reporter

    Depositor

    Zsuzsanna Izsvak

    Article

    Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

    Insert

    LTR7-GFP
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR7

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Use

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

    Plasmid

    #226577

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    I178A, L182A, L186A, V190A

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

    Plasmid

    #226578

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L4440_BioBrick-SCR-sgRNA-A

    Plasmid

    #177811

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    Scrambled sgRNA targeting sequences
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge