We narrowed to 17,206 results for: puro

1. EZH2 E10.3 gRNA

   Plasmid

   #90685

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EZH2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   EZH2 (Guide Designation E10.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PresentER-NLVPMVATV (GFP)

   Plasmid

   #102947

   Purpose

   PresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)

   Depositor

   David Scheinberg

   Article

   Gejman et al Cancer Immunol Res. 2020 May;8(5):672-68

   Insert

   NLVPMVATV
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBLO 62.5

   Plasmid

   #123124

   Purpose

   Human expression vector for planctomyces CasX and sgRNA

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. F40 tension sensor

   Plasmid

   #101252

   Purpose

   Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-F40-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRXIP Lamp1-Venus

   Plasmid

   #89937

   Purpose

   Expresses Lamp1 tagged with Venus in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

   Insert

   rat Lamp1 (Lamp1 Rat)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2 EGFP sgRNA2

   Plasmid

   #164933

   Purpose

   Expresses EGFP sgRNA2 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DKC1 F1.3 gRNA

   Plasmid

   #90652

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DKC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DKC1 (Guide Designation F1.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pR26-CMVconst

   Plasmid

   #127373

   Purpose

   Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV enhancer/promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. DKC1 F2.3 gRNA

    Plasmid

    #90653

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F2.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDH-EF1-FHC-POLQ-DM

    Plasmid

    #64880

    Purpose

    lentiviral expression of human POLQ double mutant (in POL and HDL domains)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M and D2330A,Y2331A

    Promoter

    EF1alpha

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIP-SHMY-PAUF

    Plasmid

    #182645

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    Pancreatic adenocarcinoma upregulated factor (PAUF)
    

    Use

    Retroviral

    Tags

    His6-tag, N-terminal ER targeting sequence of hem…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCh-LgBiT-2A-EGFP-Vin-PILATeS

    Plasmid

    #216761

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KIF20A D5.2 gRNA

    Plasmid

    #90718

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF20A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF20A (Guide Designation D5.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BRCA1 A10.2 gRNA

    Plasmid

    #90549

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRetrosuper Fbw7 shRNA-1

    Plasmid

    #15660

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    Fbw7 shRNA (FBXW7 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pB-Ric8b-Integration

    Plasmid

    #129457

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    Ric8b (Ric8b Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2-7xTcf-NLS-CNL

    Plasmid

    #65712

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

    Plasmid

    #122083

    Purpose

    Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-LaminB1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of LaminB1

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p7140 pHAGE-P-CMVt-N-HA-GAW-EME1

    Plasmid

    #100150

    Purpose

    Expresses EME1

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    EME1 (EME1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1-Pur-CAG-hM3Dq-mCherry

    Plasmid

    #80948

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM3Dq-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SMC6 G4.4 gRNA

    Plasmid

    #90899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G4.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SMC6 G3.4 gRNA

    Plasmid

    #90898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G3.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid

    #117720

    Purpose

    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    mAID-nanobody
    IBB-mCherry
    

    Use

    Tags

    importin-beta bining domain (IBB) and triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV and None, IRES-driven

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQCXIP-GFP-LmnB1

    Plasmid

    #164249

    Purpose

    retroviral plasmid for GFP-LmnB1

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    LMNB1 (LMNB1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRetrosuper Fbw7 shRNA-2

    Plasmid

    #15661

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    Fbw7 shRNA (FBXW7 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

27. pCEFL EGFP-TEADi

    Plasmid

    #140144

    Purpose

    TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    EGFP TEADi
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. shTCF7L2 # 1

    Plasmid

    #42557

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pLEX_305-N-dTAG_OGT

    Plasmid

    #155196

    Purpose

    N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    lacking N-terminal Met

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

    Plasmid

    #186699

    Purpose

    Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAVS1-Pur-CAG-hM4Di-mCherry

    Plasmid

    #80947

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM4Di-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX317-hcRED

    Plasmid

    #115440

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    hcRED
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FEN1 C10.3 gRNA

    Plasmid

    #90689

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C10.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSCV mSin1.1 myc

    Plasmid

    #12576

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Aug 15. ():.

    Insert

    mSin1.1 (MAPKAP1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

35. pMXs-IP-EGFP-mAtg5

    Plasmid

    #38196

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLVX-NPC1(P202A/F203A)-FLAG

    Plasmid

    #164976

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P202A/ F203A

    Promoter

    EF1a

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXSIP-FLAG-ATPIF1

    Plasmid

    #204703

    Purpose

    expresses FLAG-ATPIF1 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMXs-IP-EGFP-LC3

    Plasmid

    #38195

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLEX-FHH-MTORKinaseDomain

    Plasmid

    #120708

    Purpose

    Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MTOR Kinase Domain (MTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 1967-2549

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMSCV-mCherry-Vav1

    Plasmid

    #50044

    Purpose

    Expression of mCherry-Vav1 in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    Vav1 (Vav1 Mouse)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge