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We narrowed to 31,746 results for: son

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Showing: 5541 - 5560 of 31746 results

  1. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid
    #107320
    Purpose
    Expresses SpCas9 fused to SaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to SaCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    107320-plasmid-map-sequence-id-204686

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  5. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

    Plasmid
    #78348
    Purpose
    Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
    Depositor
    Michael Elowitz
    Publication
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mCherry-T2A-rTetR-KRAB
    Use
    Synthetic Biology
    Tags
    rTetR
    Expression
    Mammalian
    Promoter
    pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    78348-plasmid-map-sequence-id-146273

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  7. mPA-GFP-MAPTau-N-10

    Plasmid
    #57146
    Purpose
    Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPTau (MAPT Human)
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57146-plasmid-map-sequence-id-263558

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  9. CoV2-Spike-D614G-Alpha

    Plasmid
    #177961
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)
    Depositor
    Melanie Ott
    Publication
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
    Expression
    Mammalian
    Mutation
    del69, 70, del144, D614G, N501Y, A570D, P681H, T7…
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    177961-plasmid-map-sequence-id-348264

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  11. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

    Plasmid
    #169912
    Purpose
    Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.
    Depositor
    Zhili Rong
    Publication
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCjCas9
    Use
    CRISPR
    Tags
    2xSV40NLS, 10XGCN4, and SV40NLS-HA
    Expression
    Mammalian
    Mutation
    D8A, H559A in cjCas9
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169912-plasmid-map-sequence-id-336049

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  12. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    160140-plasmid-map-sequence-id-317331

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  13. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  14. mCh-KIF13A*-strep

    Plasmid
    #120166
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 13A (KIF13A Human)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF13A: 1-639 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    120166-plasmid-map-sequence-id-247501

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  17. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  18. opto-E-cad GFP

    Plasmid
    #203327
    Purpose
    Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells
    Depositor
    Seraphine Wegner
    Publication
    Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Addition of Aslov2 domain at T133 of human E-cadh…
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    203327-plasmid-map-sequence-id-411099

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Showing: 5541 - 5560 of 31746 results