We narrowed to 9,184 results for: Pol;

1. GFP-LacI-p300

   Plasmid

   #179270

   Purpose

   Expresses GFP-LacI-p300 fusion plasmid

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Methods Mol Biol. 2023;2589:361-376. doi

   Insert

   E1A binding protein p300 (EP300 Human)
   

   Tags

   GFP-LacI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro-PARG shRNA

   Plasmid

   #23260

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   poly(ADP-ribose) glycohydrolase shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

3. FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

   Plasmid

   #216380

   Purpose

   Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJUMP18-lambdaRED_NOC

   Plasmid

   #127032

   Purpose

   Basic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part ?RED_NOC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac(His10-SNAP-Csk)

   Plasmid

   #177895

   Purpose

   Baculo expression of His10 tag fused to SNAPtag and human Csk

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Csk (CSK Human)
   

   Tags

   10xHis-TEV-TVMV-SNAP tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRJPaph-lacZYA

   Plasmid

   #67037

   Purpose

   Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   lacZYA
   

   Use

   Conjugative bacterial vector

   Available Since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-HiFi-nCas9n-A72

   Plasmid

   #125646

   Purpose

   micro-injection of poly-adenylated HiFi-nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   HiFi-nCas9n
   

   Use

   Rna in vitro transcription vector

   Mutation

   R691A from wild-type Cas9

   Promoter

   T3

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFlox_N-RPB1_BSD_FKBPF36V_EYFP

   Plasmid

   #163884

   Purpose

   Acute Depletion of RBP1-EYFP

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Rbp1 (Polr2a Mouse)
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcGP67A-rCL1-HormR-GAIN

   Plasmid

   #37847

   Depositor

   Axel Brunger

   Article

   Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

   Insert

   CL1 (Adgrl1 Rat)
   

   Use

   Baculovirus transfer vectors

   Tags

   8xHIS

   Mutation

   HormR and GAIN domains only (aa460–849

   Promoter

   Polyhedrin

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

10. K20‐scFv‐pSMBP2

    Plasmid

    #159940

    Purpose

    To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

    Depositor

    Sandra Schmid

    Article

    Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

    Insert

    anti beta-1 integrin scFv
    

    Tags

    6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. DEP-GFP

    Plasmid

    #109043

    Purpose

    Expression of the Dishevelled DEP domain

    Depositor

    Mariann Bienz

    Article

    Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

    Insert

    dishevelled segment polarity protein 2 (DVL2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Dvl2 DEP domain

    Promoter

    CMV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBacPAK p110a RBD DM

    Plasmid

    #55723

    Purpose

    His3 GST tagged RBD double mutant p110 alpha T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Tags

    His3 GST

    Mutation

    T207D, K227A

    Promoter

    polyhedrin promoter

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pOCC267

    Plasmid

    #118887

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; HALO tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. ptdEos-CLC

    Plasmid

    #38009

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Inserts

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    Eos FP, tandem dimer
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRJPaph-gusA

    Plasmid

    #67036

    Purpose

    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    

    Use

    Conjugative bacterial vector

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSG5-Cyp26a1

    Plasmid

    #13457

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Cyp26a1 (Cyp26a1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pU6-26 (GB1001)

    Plasmid

    #68208

    Purpose

    Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtU6-26 promoter
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJet-CMV-CD5Flag2-bglpA

    Plasmid

    #89705

    Purpose

    Expression plasmid for Flag embedded into human CD52 with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CD5Flag2-bglpA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2pA2-drl:APEX2-mCherry

    Plasmid

    #188945

    Purpose

    Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

    Depositor

    Owen Tamplin

    Article

    Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

    Insert

    drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
    

    Use

    Tol2 destination vector (attr4-r3)

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pK RagA(Q66L)-RagC

    Plasmid

    #99700

    Purpose

    Bacterial expression of RagA(Q66L)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pQE80L TriCatcher-RGDSP-MMP

    Plasmid

    #112633

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP-MMP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAG-T3-st1CAS9-pA

    Plasmid

    #131542

    Purpose

    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

    Insert

    Codon optimized st1Cas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pOCC167

    Plasmid

    #118909

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAC8-TwinStrep-3C-DrHDAC6 (40-831)

    Plasmid

    #180226

    Purpose

    Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

    Depositor

    Patrick Matthias

    Article

    Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

    Insert

    Histone deacetylase 6 (hdac6 Zebrafish)
    

    Tags

    TwinStrep-3C

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRJPaph-mTq2

    Plasmid

    #67031

    Purpose

    Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    mTurqoise2
    

    Use

    Conjugative bacterial vector

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSBL_DK193

    Plasmid

    #156424

    Purpose

    Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (Q57H)
    

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Q57H

    Promoter

    Polyhedrin

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV6M-Pak1 P13A

    Plasmid

    #12207

    Depositor

    Jonathan Chernoff

    Article

    Galisteo et al J Biol Chem. 1996 Aug 30. 271(35):20997-

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P13A: disrupts first polyproline region of Pak1

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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30. pOCC93

    Plasmid

    #118911

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Tags

    eGFP-HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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