We narrowed to 6,226 results for: ARL

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pLVX-RNaseHI-NES-EGFP

Plasmid

#196701

Purpose

Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

bacterial RNaseHI

Use

Lentiviral

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP7

Plasmid

#178004

Purpose

Expresses N-terminally mEGFP-tagged PARP7

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP7 (TIPARP Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti Lifeact-EGFP BlastR

Plasmid

#84383

Purpose

Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-Caspase 1

Plasmid

#41552

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

Caspase-1 (CASP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV immediate-early enhancer/promoter

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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NSUN6

Plasmid

#188060

Purpose

Bacterial expression plasmid for human NSUN6; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN6 (NSUN6 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-165

Plasmid

#118545

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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LV-TRE-WT mouse MyoD-T2A-dsRedExpress2

Plasmid

#60624

Purpose

Expresses WT mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

WT mouse MyoD (Myod1 Mouse)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 S22N

Plasmid

#107106

Purpose

Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 wt

Plasmid

#107104

Purpose

Lentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#244109

Purpose

Cytosolic expression of non-responsive circularly permuted Super Folder GFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

cpSFGFP

Use

AAV

Tags

HaloTag

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-EYFP

Plasmid

#231926

Purpose

Cre-on/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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