We narrowed to 64,941 results for: Cel

1. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

   Plasmid

   #222993

   Purpose

   Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-Oct3/4-IP

   Plasmid

   #15918

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. (SUMO)10

   Plasmid

   #126948

   Purpose

   Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. px458_2A_GFP_sgRNA_TIAL1

   Plasmid

   #106096

   Purpose

   CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   gRNA TIAL1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-CAG-Tfe3-pgk-hph

   Plasmid

   #48755

   Purpose

   PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selection

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Insert

   Tfe3 (Tcfe3 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

6. 3XHA-PP2A-Ba

   Plasmid

   #195229

   Purpose

   Retroviral vector containing HA tagged PP2A

   Depositor

   Keith Mostov, David Bryant

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PPP2R2A (PPP2R2A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-T7-Nla-repeat-BbsI

   Plasmid

   #180215

   Purpose

   For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC Ad VAI

   Plasmid

   #175518

   Purpose

   For mammalian expression of adenovirus VAI RNA

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   Ad VAI RNA
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   lac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH2.30_Gag-MCP-dpol-T2A-eGFP

   Plasmid

   #205538

   Purpose

   Express HIV Gag-MCP-dpol-T2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   HIV Gag-MCP-dpol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   pol frameshift sequence recoded

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

    Plasmid

    #222992

    Purpose

    Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. tagSrc-HaloTag

    Plasmid

    #179141

    Purpose

    The tag is SsrA (AANDENY). Tag is inserted after N534 of Src.

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. epi-ABEmax

    Plasmid

    #135974

    Purpose

    Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

13. pBS-KS-attB2-SA(0)-T2A-Gal4

    Plasmid

    #62899

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAM1.18_PB-SDC4-PGK-Blast

    Plasmid

    #205563

    Purpose

    Express human SDC4

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT-GEM(2)

    Plasmid

    #62894

    Purpose

    Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHRSin_hTCRβεζ

    Plasmid

    #187353

    Purpose

    Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-Brn2

    Plasmid

    #32925

    Purpose

    Retroviral expression of mouse Brn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pPy-CAG-Cre::ERT2-IRES-BSD

    Plasmid

    #48760

    Purpose

    CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Cre::ERT2
    

    Use

    Ppy

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP mCherry-ApoB-EGFP

    Plasmid

    #112859

    Purpose

    Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

    Depositor

    Silvestro Conticello

    Article

    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

    Insert

    mCherry-apoB-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FSW-HRP-V5-NLGN2

    Plasmid

    #82545

    Purpose

    Overexpression vector for V5-NLGN2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    NLGN2 (Nlgn2 Rat)
    

    Use

    Lentiviral

    Tags

    HRP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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