We narrowed to 6,088 results for: pcDNA 3.1

1. SpyCas9-AIDx

   Plasmid

   #135343

   Purpose

   Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSP14-Flag

   Plasmid

   #210346

   Purpose

   Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mH2A2-CT-MYC

   Plasmid

   #45170

   Depositor

   Brian Chadwick, Hunt Willard

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy2 (MACROH2A2, H2AFY2 Human)
   

   Tags

   HIS and MYC

   Expression

   Mammalian

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

4. bArr1-ALFA

   Plasmid

   #201496

   Purpose

   Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   bArr1-ALFA (ARRB1 Human)
   

   Tags

   ALFA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LRRK2-K1906M

   Plasmid

   #35946

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

6. Opto-GPR3

   Plasmid

   #106000

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR3 (GPR3 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. F-MED28

   Plasmid

   #15368

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

   Insert

   MED28 (MED28 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. ABEMax nSaCas9

   Plasmid

   #135364

   Purpose

   Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax nSaCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)

   Plasmid

   #183254

   Purpose

   Expression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector

   Depositor

   Alexander McLellan

   Article

   Muhsin-Sharafaldine et al Oncotarget. 2016 Aug 30;7(35):56279-5629

   Insert

   Tissue factor (F3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmyc-GFP-TNRC6A-(935-1026)-NES-mut

    Plasmid

    #42021

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa935-1026, F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Kif1A(1-489)-DHFR-myc

    Plasmid

    #117833

    Purpose

    Constitutively active Kinesin-3 motor tagged with DHFR and myc

    Depositor

    Thomas Schwarz

    Article

    Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

    Insert

    Kif1A (1-489) (Kif1a Mouse)
    

    Tags

    DHFR and myc

    Mutation

    only aa 1-489 (motor domain)

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

    Plasmid

    #210504

    Purpose

    expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
    

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opto-GPR27

    Plasmid

    #106013

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR27 (GPR27 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRD398

    Plasmid

    #163105

    Purpose

    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mCherry_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

    Plasmid

    #42040

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa457-532, aa716-772 and aa804-924, F951A…

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTOPO-MCL1S159A

    Plasmid

    #21606

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 S159A (MCL1 Human)
    

    Expression

    Mammalian

    Mutation

    Serine 159 mutated to Alanine, abolishing phosph…

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

17. Opto-GPR150

    Plasmid

    #106050

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR150 (GPR150 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-MED9

    Plasmid

    #15407

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED9 (Med9 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pRD190

    Plasmid

    #163100

    Purpose

    Expression of mEos3.2 in eukaryotes to stain the nuclear compartment

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mEos3.2_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTE3987

    Plasmid

    #137716

    Purpose

    Expresses dLbCas12a-RVRR-BE in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dLbCas12a-RVRR-BE
    

    Tags

    6xHis, APOBEC-1, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    G532A, K538V, Y542R, K595R

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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