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EZ-Tet-pLKO-Puro

Plasmid

#85966

Purpose

Tet/Dox inducible shRNA lentivirus with puromycin selection

Depositor

Cindy Miranti

Publication

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pCAGEN

Plasmid

#11160

Purpose

Mammalian expression vector for cloning and expressing your gene under the CAG promoter

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

modified multiple cloning sites

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

100 Articles

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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Publication

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

TRE

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pLenti CMV Hygro DEST (w117-1)

Plasmid

#17454

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

56 Articles

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Publication

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

94 Articles

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pimEJ5GFP

Plasmid

#44026

Depositor

Jeremy Stark

Publication

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EJ5GFP egfp-based chromosomal break reporter

Expression

Mammalian

Mutation

pCAGGS promoter and eGFP separated by pgkPURO cas…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

98 Articles

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DsRed-rab11 WT

Plasmid

#12679

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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GFP-Ub

Plasmid

#11928

Purpose

Used for in vivo monitoring of ubiquitin localization.

Depositor

Nico Dantuma

Publication

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ubiquitin (UBC Human)

Tags

GFP

Expression

Mammalian

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mouse U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pX330S-2-PITCh

Plasmid

#63670

Purpose

Expresses Cas9 nuclease and the PITCh-gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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