We narrowed to 5,867 results for: nls

1. Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

   Plasmid

   #82024

   Purpose

   Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   mKate2-2xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG-TurboID-3XNLS

   Plasmid

   #209392

   Purpose

   Transiently expressing TurboID fused with 3X nuclear localization signal (NLS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-3XNLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_SBE4:mScarlet-I-NLS

   Plasmid

   #154961

   Purpose

   encodes transcriptional reporter of SMAD2/3

   Depositor

   Ewa Bielczyk-Maczynska, Joshua Knowles

   Article

   Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

   Insert

   mScarlet
   

   Use

   Piggybac

   Tags

   NLS-PEST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUGW NLS-Cre

   Plasmid

   #183425

   Purpose

   Lentiviral Cre vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   NLS-Cre
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. V5-LOV-Turbo-NLS

   Plasmid

   #199663

   Purpose

   expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NLS and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTagBFP2-PCNA-19-SV40NLS-4

   Plasmid

   #55320

   Purpose

   Localization: Nucleus, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   3xSV40 NLS and mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. epB_UbC_CymRV5-nls-Halox2_DEx4

   Plasmid

   #119907

   Purpose

   ePiggyBac mammalian expression plasmid for CymR-Halo fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   CymRV5-nls-Halox2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-MCP-AP_pLX304

   Plasmid

   #120918

   Purpose

   Lentiviral vector expressing NLS-MCP-AP in mammalian nucleus

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   NLS-MCP-AP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCS:GFPnls-T2a-ER-Cre-ER

    Plasmid

    #200106

    Purpose

    Vector for LFNG

    Depositor

    Prayag Murawala

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+ GCamp6-3NLS

    Plasmid

    #165401

    Purpose

    Expresses a nuclear localized genetically encoded calcium reporter.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    Nuclear Localization Signal
    

    Use

    Avian, xenopus, zebrafish

    Tags

    GCamp6

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNLS-TetR-GFP

    Plasmid

    #103838

    Purpose

    Flourescent marker for tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    NLS-TetR (tetR )
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN1-NLS-pHlu

    Plasmid

    #129320

    Purpose

    pH measurement, nucleus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    pHluorin (from H. Imamura)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

    Plasmid

    #32628

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Sce
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN1-NLS-QUE37C

    Plasmid

    #129323

    Purpose

    ATP measurement, nucleus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-enCas12a-2xNLS

    Plasmid

    #209021

    Purpose

    Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    enCas12a-2xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R/K548R

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. V5-BS2-NLS (Nucleoplasmin)

    Plasmid

    #220342

    Purpose

    Mammalian expression vector BS2 in nucleus

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    NLS (Nucleus localization sequence) and V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. plenti-Lb-Cas12a-2xNLS

    Plasmid

    #155046

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4xNLS-pMJ915v2-sfGFP

    Plasmid

    #88921

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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