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pC007 - pRFP constitutive expression
Plasmid#79156PurposeRFP constitutively expressedDepositorInsertRFP
UseTagsExpressionMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-Stx7
Plasmid#45921DepositorInsertsyntaxin 7 (STX7 Human)
UseRetroviralTagsGFPExpressionMutationPromoterCMVAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPL5071_TEF1*-Cre_URA3
Plasmid#60930PurposeConstitutive expression vector for Cre-recombinase containing URA3 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX854
Plasmid#62886PurposeC-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).DepositorInsertSpCas9 (aa573-1368)
UseCRISPRTagsFKBP12, NLS SV40, and NLS nucleoplasminExpressionMammalianMutationPromoterCBhAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Orange_CaMBI_300
Plasmid#124095Purposeorange calcium-modulated bioluminescent indicator, 300nMDepositorInsertOrange CaMBI 300
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-Stx17TM
Plasmid#45910DepositorInsertSyntaxin 17 (STX17 Human)
UseRetroviralTagsGFPExpressionMutationdeleted amino acids 1-228PromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-VAMP8
Plasmid#45912DepositorInsertvesicle-associated membrane protein 8 (VAMP8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKAR4α
Plasmid#119919PurposeMammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imagingDepositorInserttAKAR4α
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Blue_CaMBI
Plasmid#124097PurposeBlue calcium-modulated bioluminescent indicatorDepositorInsertBlue CaMBI
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Green_CaMBI
Plasmid#124096PurposeGreen calcium-modulated bioluminescent indicatorDepositorInsertGreen CaMBI
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMHG-W57* reporter
Plasmid#72850PurposeGFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT2h
Plasmid#208709PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-FRB-Fis1tail
Plasmid#220080PurposeFRB targeted to the mitochondria with the tail of the mitochondrial outer membrane protein Fis1 with an iRFP fluorophoreDepositorInsertFRB-Fis1tail
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl
Plasmid#36400DepositorInsertCrkl (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-Stx18
Plasmid#45918DepositorInsertsyntaxin 18 (STX18 Human)
UseRetroviralTagsGFPExpressionMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PTPN11 in pENTR
Plasmid#216604Purposegateway cloning donor vector containing PTPN11DepositorInsertPTPN11 (PTPN11 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC008 - pBR322 with tetR-inducible RFP
Plasmid#79157Purposeinducible RFP expression vectorDepositorInsertTetR + RFP
UseTagsExpressionMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMyc-CMV1
Plasmid#53582Purposemammalian expression vector with Myc tagDepositorTypeEmpty backboneUseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2-TagRFPT.zf1
Plasmid#61390PurposemRNA synthesis of TagRFPT codon modified for zebrafishDepositorInsertTagRFPT.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
ANO10 (TMEM16KA-c001)
Plasmid#110267PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMEM16K (ANO10 Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKPY514
Plasmid#62598PurposeEncodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) ControlDepositorInsertThr251Gly-EcPheRS
UseTags6xHisExpressionBacterialMutationThr251GlyPromoterT5Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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MKbpAII
Plasmid#91689PurposeMMTV-LTR promoter/enhancer with rabbit beta-globin intron and bovine growth hormone poly-A sequence to enhance transgene expressionDepositorInsertMMTV LTR cloned into KbpA vector
UseTagsExpressionMammalianMutationPromoterMMTV LTRAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPL5628_TEF1*-Cre_MET15
Plasmid#60933PurposeConstitutive expression vector for Cre-recombinase containing MET15 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
ERAP1
Plasmid#39174PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertERAP1 (ERAP1 Human)
UseBaculovirus expressionTagsTEV-His10-FlagExpressionMutationPromoterpolyhedrinAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pADtet-His6-MscL
Plasmid#83373PurposeExpresses wild type MscL in mammalian cellsDepositorInsertMechanosensitive channel of large conductance (mscL E. coli)
UseAdenoviralTagsHis6 tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRK5_tAKARα-T391A
Plasmid#119920PurposeMammalian expression of the tAKARα sensor with T391A mutationDepositorInserttAKARα-T391A
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cer.zf1
Plasmid#61389PurposemRNA synthesis of cerulean codon modified for zebrafishDepositorInsertCer.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTAPmyc-T2A-tagRFP
Plasmid#140278PurposeBackbone for making a mammalian expression vector of the protein of interestDepositorInsertEmpty backbone
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNeurog2-Luciferase reporter
Plasmid#64126PurposePhotoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.DepositorInsertNeurog2 promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterNeurog2Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3
Plasmid#110532PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
