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pFS_0378_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2_RC

Plasmid

#116990

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0375_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1

Plasmid

#116987

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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SYNJ1-145_pCSdest

Plasmid

#53810

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SYNJ1-145 (SYNJ1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
CMYA5/pDEST15

Plasmid

#98106

Purpose

Express CMYA5 (aa 4039 – 4069) with a N-terminal GST tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CMYA5T2/pDEST17

Plasmid

#98118

Purpose

Express CMYA5 (aa 4039 – 4069) Leu allele (4063) witha N-terminal 6x-His tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Tags

6X-His

Expression

Bacterial

Mutation

rs10043986 (NP_705838.3:p.Pro4063Leu)

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CMYA5/pDEST17

Plasmid

#98115

Purpose

Express CMYA5 (aa 4039 – 4069) with a N-terminal 6x-His tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Tags

6X-His

Expression

Bacterial

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBW755_pCAG-pDest-U5-UX

Plasmid

#89507

Purpose

pCAG destination vector (U5-UX)

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1716_hU6-pDest-U1-UX

Plasmid

#87579

Purpose

hU6 destination vector

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST27-WFS1

Plasmid

#79276

Purpose

Mammalian expression of WFS1 with an N-terminal GST

Depositor

Rita Shiang

Insert

WFS1 (WFS1 Human)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits
POLN delP_pDEST17

Plasmid

#64886

Purpose

bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail

Depositor

Richard Wood

Article

Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5

Insert

POLN (POLN Human)

Tags

Flag and His

Expression

Bacterial

Mutation

with a short truncation of the C-terminal proline…

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL2

Plasmid

#67459

Purpose

Mammalian expression of hArl2 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4

Plasmid

#67461

Purpose

Mammalian expression of hArl4 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Expression

Mammalian

Mutation

bp 105 A to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5A

Plasmid

#67462

Purpose

Mammalian expression of hArl5 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5B

Plasmid

#67463

Purpose

Mammalian expression of hArl5b with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5B (ARL5B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL6

Plasmid

#67464

Purpose

Mammalian expression of hArl6 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4D

Plasmid

#67468

Purpose

Mammalian expression of hArl4D with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL14

Plasmid

#67469

Purpose

Mammalian expression of hArl14 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL11

Plasmid

#67470

Purpose

Mammalian expression of hArl11 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Expression

Mammalian

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF3

Plasmid

#67454

Purpose

Mammalian expression of hArf3 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF5

Plasmid

#67456

Purpose

Mammalian expression of hArf5 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Expression

Mammalian

Mutation

bp 477 T to C; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL6

Plasmid

#67380

Purpose

Expression of native hArl6 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL4C

Plasmid

#67381

Purpose

Expression of native hArl4c in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL8A

Plasmid

#67382

Purpose

Expression of native hArl8 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL4D

Plasmid

#67384

Purpose

Expression of native hArl4D in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL14

Plasmid

#67385

Purpose

Expression of native hArl14 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Expression

Bacterial

Mutation

bp 172 C to T; aa L58F

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL11

Plasmid

#67386

Purpose

Expression of native hArl11 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Expression

Bacterial

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARFRP1

Plasmid

#67387

Purpose

Expression of native hArfrp in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-SARA

Plasmid

#67389

Purpose

Expression of native hSara in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF1

Plasmid

#67369

Purpose

Expression of native hArf1 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF3

Plasmid

#67370

Purpose

Expression of native hArf3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF4

Plasmid

#67371

Purpose

Expression of native hArf4 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF5

Plasmid

#67372

Purpose

Expression of native hArf5 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Expression

Bacterial

Mutation

bp 477 T to C; silent mutation

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARF6

Plasmid

#67373

Purpose

Expression of native hArf6 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL1

Plasmid

#67374

Purpose

Expression of native hArl1 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL2

Plasmid

#67375

Purpose

Expression of native hArl2 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL4

Plasmid

#67377

Purpose

Expression of native hArl4 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL5A

Plasmid

#67378

Purpose

Expression of native hArl5 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Expression

Bacterial

Mutation

bp 70 C to T; silent mutation

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestGFP_RBPMS2

Plasmid

#65747

Purpose

expresses GFP-tagged RBPMS2 in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

GFP

Expression

Mammalian

Mutation

S13N mutation in RBPMS2

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_KDR

Plasmid

#65091

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed sequence to render plasmid resistant to s…

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestMYC_RBPMS2

Plasmid

#65668

Purpose

MYC expression vector with RBPMS2

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS2 (RBPMS2 Human)

Tags

MYC

Expression

Mammalian

Mutation

introduced HindIII site to exchange FLAG-HA tag w…

Promoter

CMV, 2x Tet

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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