We narrowed to 5,482 results for: pcas

1. Cav2.1 HA pCAGGS

   Plasmid

   #206076

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS9-TPC

   Plasmid

   #61478

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PcUbi4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA-SpCas9-g5

   Plasmid

   #80788

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-NL1AB

   Plasmid

   #15262

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Neuroligin 1 with inserts A and B (Nlgn1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA-SpCas9-g6

   Plasmid

   #80789

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-T3-hCAS-pA

   Plasmid

   #48625

   Purpose

   Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii, Kunihiko Naito

   Article

   Fujii et al Nucleic Acids Res. 2013 Aug 30.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVNP2.0-SpCas9

   Plasmid

   #206880

   Purpose

   Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Insert

   SpCas9 (cas9 )
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV_Efs_hSpCas9_NLS_FLAG-SV40

   Plasmid

   #97307

   Purpose

   AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   AAV and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-delta90GFP

    Plasmid

    #26645

    Depositor

    Anjen Chenn

    Article

    Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

    Insert

    delta90 beta-catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deletion of first 90 aa of beta catenin

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAP-BAC

    Plasmid

    #120229

    Purpose

    TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG_EGFP-RandE3

    Plasmid

    #79870

    Purpose

    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-YTB

    Plasmid

    #26721

    Depositor

    Edward Callaway

    Article

    Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

    Insert

    venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Rabies virus (strain SAD B19), Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCasperAUG-Gal4-X

    Plasmid

    #8378

    Depositor

    Leslie Vosshall

    Article

    Vosshall et al Cell 2000 Jul 21;102(2):147-59.

    Insert

    Yeast Gal4 (GAL4 Budding Yeast, fused to first ~30 amino acids of Drosophila ADH)
    

    Use

    Drosophila p-element vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pCAT112TAG-SepT

    Plasmid

    #34624

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    tRNA^Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Cre

    Plasmid

    #125574

    Purpose

    Expresses Cre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-NCad-B1

    Plasmid

    #191667

    Purpose

    ddFP-B1-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddFP-B1

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-VPS34

    Plasmid

    #203564

    Purpose

    Mammalian expression of VPS34

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    VPS34 (PIK3C3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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