We narrowed to 83,221 results for: ELL

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TetO-FUW-NfiB

Plasmid

#64900

Purpose

Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

NfiB (Nfib Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT WT

Plasmid

#84924

Purpose

luciferase reporter for TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G228A

Plasmid

#84926

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G228A

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G250A

Plasmid

#84925

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G250A

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pINDUCER21-ERG

Plasmid

#51301

Purpose

Expression of ERG after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-Bi-DREADD

Plasmid

#159457

Purpose

AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

Bi-DREADD

Expression

Mammalian

Mutation

fusion protein of hM3Dq-mCherry and HA-KORD seper…

Promoter

CAG

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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Nsp3 -EGFP

Plasmid

#165108

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2

Plasmid

#174491

Purpose

bacterial expression of human SEPT2

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pLCKO2

Plasmid

#125518

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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Nsp8-EGFP

Plasmid

#165113

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp8 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pVax1-hVEGF165

Plasmid

#74466

Purpose

expression of hVEGF165 in mammalian cells

Depositor

Loree Heller

Article

Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

Insert

vascular endothelial growth factor 165 (VEGFA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-TRIM21

Plasmid

#207838

Purpose

Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

TRIM21 (TRIM21 Human)
mCherry
hIgG1-FC
VHH-GFP4

Tags

weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Nsp4-mCherry

Plasmid

#165132

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-602_NY-ESO-1_TCR_RFP

Plasmid

#207483

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP, NY-ESO-1_TCR

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

Plasmid

#205444

Purpose

human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-HLA-E SCT-hCD55

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGBKT7_AvrSr62-5

Plasmid

#233532

Purpose

Expresses AvrSr62-5 in yeast

Depositor

Brande Wulff

Article

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

AvrSr62-5

Tags

GAL4 BD, Myc tag

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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