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FLAG-PIWI(aa478-860)

Plasmid

#21543

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa478-860

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pSW609 - N001

Plasmid

#115993

Purpose

intermediate vector for GreenGate cloning method, contains modules from A-F

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Unspecified

Tags

HDEL and SP(ER)

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-MGD

Plasmid

#33033

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Tags

6xMyc

Expression

Mammalian

Mutation

MGD = amino acid substitutions M383I, G384S and D…

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
FUG-T2A-Cas9

Plasmid

#75346

Purpose

Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

Depositor

Bryan Luikart

Article

Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

GFP (via t2a)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-mir20

Plasmid

#24709

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir-20 (MIR20A Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hEZH2

Plasmid

#112543

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Zhou et al Mol Ther. 2014 Feb;22(2):378-389. doi: 1

Insert

EZH2 (EZH2 Human)

Use

Luciferase

Available Since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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POT4-RFP

Plasmid

#65320

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pKMV-VioE

Plasmid

#65351

Purpose

systhesis of vioE gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioE

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA scaffold-spacer-human U6

Plasmid

#214697

Purpose

provide "sgRNA scaffold-spacer-human U6" fragment

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

sgRNA scaffold-spacer-human U6

Use

CRISPR

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BFRF3

Plasmid

#195778

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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Flag Snail 4SA

Plasmid

#16220

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

4SA: S108A, S112A, S116A, S120A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
DsRed2_pLENTI-CAG-IRES-GFP

Plasmid

#177010

Purpose

Mammalian lentiviral expression vector encoding DsRed2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-BKRF3

Plasmid

#195790

Purpose

Gateway Entry Clone of Epstein-Barr virus BKRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BKRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b GFP-Rab10 Q68LHis

Plasmid

#186015

Purpose

GFP-Rab10 Q68LHis

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

GFP-Rab10 Q68LHis (RAB10 Human)

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF2

Plasmid

#195800

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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LLP779_αGCN4-VP64-CO

Plasmid

#211772

Purpose

SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-VP64

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect VP64 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pKMV-VioA

Plasmid

#65347

Purpose

systhesis of vioA gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioA

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-PAZ(aa1-480)

Plasmid

#21529

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa1-480

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pMIR-Dsred-IRES-His Keap

Plasmid

#62457

Purpose

expresses his tagged human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

HisTag

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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