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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 22,283 results for: fus

Showing: 8401 - 8430 of 22283 results

  1. pAM5081

    Plasmid
    #85107
    Purpose
    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    YFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85107-plasmid-map-sequence-id-180104

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  2. RGD-I27-CC

    Plasmid
    #85393
    Purpose
    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85393-plasmid-map-sequence-id-172696

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  3. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86972-plasmid-map-sequence-id-173929

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  4. p1G108CFa

    Plasmid
    #85089
    Purpose
    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…
    Promoter
    T7 promoter
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    85089-plasmid-map-sequence-id-164483

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  7. pET30-GBF-E6

    Plasmid
    #21696
    Depositor
    James Baleja
    Article
    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.
    Insert
    protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
    Use
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Promoter
    Available since
    July 28, 2009
    Availability
    Academic Institutions and Nonprofits only

  10. pS-sg:GFP

    Plasmid
    #196296
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196296-plasmid-map-sequence-id-389821

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  16. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja1 (Gja1 Rat)
    Use
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69007-plasmid-map-sequence-id-121423

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  19. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

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  22. pPlatTET-gRNA2

    Plasmid
    #82559
    Purpose
    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.
    Depositor
    Izuho Hatada
    Article
    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103
    Insert
    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    82559-plasmid-map-sequence-id-207901

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  23. pCW-IRF4-Puro

    Plasmid
    #123323
    Purpose
    Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1
    Insert
    IRF4 (IRF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    Doxycycline inducible minimal CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123323-plasmid-map-sequence-id-239610

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  26. PM-RA

    Plasmid
    #211705
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    PM-targetted RA (Lck Synthetic)
    Use
    Tags
    RA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211705-plasmid-map-sequence-id-419227

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  30. pET30a-His6-LOXCAT

    Plasmid
    #140084
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140084-plasmid-map-sequence-id-311164

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Showing: 8401 - 8430 of 22283 results