We narrowed to 30,619 results for: ica

1. RNF168-Halo HRD

   Plasmid

   #207080

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpyCatcher002-oPent

   Plasmid

   #124664

   Purpose

   Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oPent
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZmUBQ:GW

   Plasmid

   #132358

   Purpose

   Expression of CDS from the Zea mays ubiquitin promotor

   Depositor

   Paul Schulze-Lefert

   Article

   Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

   Type

   Empty backbone

   Use

   Tags

   YFP

   Expression

   Plant

   Mutation

   Promoter

   pZmUBQ

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-Sec16B

   Plasmid

   #66607

   Purpose

   Expresses Sec16B as a GFP fusion

   Depositor

   David Stephens

   Article

   Budnik et al Sci Rep. 2011;1:77. doi: 10.1038/srep000

   Insert

   Sec16B (SEC16B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAC-GGPPipi

   Plasmid

   #53280

   Purpose

   Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   crtE, idi
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. OSCA1.1

   Plasmid

   #136592

   Purpose

   Expresses the mechanosensitive channel OSCA1.1 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   OSCA1.1 (AT4G04340 Mustard Weed)
   

   Use

   Tags

   pIRES2-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4046899

   Plasmid

   #145608

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCMV4-Tax(M47)

   Plasmid

   #23286

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   Tax(M47)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L319R L320S

   Promoter

   Available since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pCS2_Renilla_luc-60A

   Plasmid

   #200397

   Purpose

   A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRL_U2AF1_S34Y_mCherry

    Plasmid

    #84018

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34Y

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-FRT-GFP-mCherry-3pGW

    Plasmid

    #53965

    Purpose

    Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

    Inserts

    GFP
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD-hfYFP

    Plasmid

    #186522

    Purpose

    Hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    araB

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-Myc-GRM1-IRES-CyRFP1

    Plasmid

    #195355

    Purpose

    mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    GRM1-IRES-CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Calreticulin-SuperNova-KDEL

    Plasmid

    #131857

    Purpose

    Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Use

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJIT163-2NLSCas9

    Plasmid

    #53064

    Purpose

    Expression of rice codon-optimized Cas9 in plant cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    Cas9
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Rice codon optimized

    Promoter

    2x35S

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFW5000

    Plasmid

    #224214

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBWB419

    Plasmid

    #140637

    Purpose

    RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXL004-ishRNA-scramble

    Plasmid

    #36311

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of scramble
    

    Use

    Lentiviral and RNAi

    Tags

    EF1a-TetR-IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. [pSK104] pET28 SLC38A9N-His

    Plasmid

    #136142

    Purpose

    bacterial expression of SLC38A9 N term fragment (1-119)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    SLC38A9 N term fragment (1-119) (SLC38A9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJAK112

    Plasmid

    #167280

    Purpose

    CodA-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGBW-m4046852

    Plasmid

    #145584

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. actc1b-mKate2-CaaX(tK)

    Plasmid

    #109488

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-CaaX(tK)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR R4 ATCC LacZ GCTT

    Plasmid

    #173668

    Purpose

    Destination vector for Golden Gate Cloning assemblies, blue-white screening

    Depositor

    Eric Marois

    Article

    Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAP003l2

    Plasmid

    #209748

    Purpose

    RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

    Depositor

    Ahmed Badran

    Article

    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

    Insert

    NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-U-TVA950

    Plasmid

    #115236

    Purpose

    Lentiviral transfer plasmid for expression of TVA950

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TVA950
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SRF WT pGEX4T

    Plasmid

    #121100

    Purpose

    Expression of SRF as a GST fusion protein

    Depositor

    Paul Herring

    Article

    Herring et al J Biol Chem. 2001 Apr 27;276(17):14482-9

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HA-UBE2E1 C131A in pcDNA3.1

    Plasmid

    #118214

    Purpose

    Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    UBE2E1 (UBE2E1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed cysteine 131 to alanine

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSB700 lenti gRNA Puro_zhang2.0

    Plasmid

    #167919

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT3TS_Transposase

    Plasmid

    #109768

    Purpose

    Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Tol2 Transposase
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28-2H12.D11

    Plasmid

    #221145

    Purpose

    His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TagBFP2-C1-SACM1LdeltaTMD-Fis1tail

    Plasmid

    #220078

    Purpose

    SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    SACM1l-Fis1tail (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deltaTMD

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EGFP-1-SAC1deltaTMD-Fis1tail

    Plasmid

    #220077

    Purpose

    SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    SACM1l-Fis1tail (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deltaTMD

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mEmerald-MCM7-C-19

    Plasmid

    #54173

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM7
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

35. mEmerald-MCM5-C-19

    Plasmid

    #54169

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM5
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

36. PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

    Plasmid

    #204497

    Purpose

    Expresses rtTA and Neomycin resistance genes in mammalian cells

    Depositor

    Wataru Fujii

    Article

    Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

    Insert

    rtTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mEmerald-MCM3-C-19

    Plasmid

    #54165

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM3
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

38. p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B

    Plasmid

    #100160

    Purpose

    Expresses PRKAR2B

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    PRKAR2B (PRKAR2B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV.GFAP.iGABASnFR.F102G

    Plasmid

    #112173

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102G

    Promoter

    GFAP

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge