We narrowed to 19,111 results for: INO

1. pcDNA3.1(+)-FluoSTEP-JNKAR

   Plasmid

   #181857

   Purpose

   Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-JNKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-FluoSTEP-AktAR

   Plasmid

   #181856

   Purpose

   Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AktAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-hCas9-T2A-mStrawberry

   Plasmid

   #187224

   Purpose

   Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Inserted T2A-mStrawberry at 3' terminal of M…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS848∙CsR

   Plasmid

   #199240

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EFS-FlpO

   Plasmid

   #191509

   Purpose

   Lentiviral Flp Recombinase driven by the EFS promoter

   Depositor

   Eric Snyder

   Article

   Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

   Insert

   flp recombinase (flp )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/EFS promoter inserted

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-eGFP-SynaptoZip

   Plasmid

   #122525

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 GABARAPL1 WT

   Plasmid

   #123083

   Purpose

   Gateway entry clone encoding human GABARAPL1 wild-type

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-CIBN-EGFP-KRasCT

   Plasmid

   #178527

   Purpose

   Expresses CIBN-EGFP-KRasCT in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CIBN-EGFP-KRasCT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO mouse ULK2 shRNA 93

   Plasmid

   #27635

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse ULK2 shRNA 93
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pDGB1alpha2_SulR

    Plasmid

    #186425

    Purpose

    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas3-Sm

    Plasmid

    #208001

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-Vps4(101-437)-Hcp1

    Plasmid

    #87737

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SBP-ST*-GFP

    Plasmid

    #163668

    Purpose

    Expresses SBP tagged partial length ST in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    

    Use

    Tags

    GFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    This chimera contains the first 113 amino acids o…

    Promoter

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

    Plasmid

    #181859

    Purpose

    Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    cp-PKN-mRuby2-GFP(1-0)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-MARS-p85(iSH2)

    Plasmid

    #205236

    Purpose

    Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

    Depositor

    Jeremy Baskin

    Inserts

    PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
    p85(iSH2) (Pik3r1 Mouse)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

    Plasmid

    #200475

    Purpose

    Lentiviral vector expressing gRNA targeting GFP

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5 MEKK4alpha KD

    Plasmid

    #12188

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Kinase dead: K1361M

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pCDF1-ERBB4 (kinase dead)

    Plasmid

    #29544

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Promoter

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

19. shPBRM1 #122-Tet-pLKO-puro

    Plasmid

    #107401

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223 GABARAPL2 WT

    Plasmid

    #123086

    Purpose

    Gateway entry clone encoding human GABARAPL2 wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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