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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,439 results for: eng

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Showing: 5621 - 5640 of 14439 results

  3. pPI227

    Plasmid
    #113231
    Purpose
    safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP
    Depositor
    Rob Kay
    Publication
    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LifeAct
    Use
    Safe haven knock-in plasmid for the act5 locus,li…
    Tags
    GFP
    Promoter
    act5
    Available since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113231-plasmid-map-sequence-id-217854

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  4. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

    Plasmid
    #98976
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 1 and Cas9(N863A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98976-plasmid-map-sequence-id-199220

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  5. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid
    #98972
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 1 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98972-plasmid-map-sequence-id-199211

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  11. PX386 Lactobacillus farciminis Cas9

    Plasmid
    #68323
    Purpose
    Lactobacillus farciminis Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LfCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68323-plasmid-map-sequence-id-119538

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  12. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid
    #68330
    Purpose
    Gluconacetobacter diazotrophicus Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68330-plasmid-map-sequence-id-119553

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  16. C25-pLAC

    Plasmid
    #45283
    Purpose
    lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide
    Depositor
    Gordon Laurie
    Publication
    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.
    Insert
    pLAC-C25 (LACRT Human)
    Use
    Protein expression vector
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    contains aa20-113 of lacritin
    Promoter
    T7
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    45283-plasmid-map-sequence-id-63156

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Showing: 5621 - 5640 of 14439 results