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Showing: 5661 - 5680 of 79192 results

  4. Epac-S-H187

    Plasmid
    #170348
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170348-plasmid-map-sequence-id-336137

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  10. Dead-Laconic/pcDNA3.1(-)

    Plasmid
    #118627
    Purpose
    To express in mammalian cells a lactate FRET sensor negative control.
    Depositor
    Luis Felipe Barros
    Publication
    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dead-Laconic
    Expression
    Mammalian
    Mutation
    Change Histidine 151 to Aspartic Acid of Escheric…
    Promoter
    CMV Promoter
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118627-plasmid-map-sequence-id-231761

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  11. pcDNA3-ExRai-AktAR

    Plasmid
    #118462
    Purpose
    cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AktAR
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    118462-plasmid-map-sequence-id-230151

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  12. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86612-plasmid-map-sequence-id-168849

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Showing: 5661 - 5680 of 79192 results