We narrowed to 9,954 results for: rras

1. pCK007 pME KalTa4

   Plasmid

   #195965

   Purpose

   Gateway pME Gal4 variant

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   KalTA4 (Gal4)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLmoCascade-IL1RNcrRNA

   Plasmid

   #126487

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   Lmo IL1RN cr03
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. TF-bio-His

   Plasmid

   #52184

   Purpose

   Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TF (TF Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

   Plasmid

   #73475

   Purpose

   Encodes the G protein to enable transsynaptic RAV infection

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   H2B-GFP-P2A-N2c(G)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Plasmid

   #163074

   Purpose

   Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Grx1-roGFP1-iEER
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ10871

   Plasmid

   #183962

   Purpose

   U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   dCas12a(wildtype)
   poly-crRNA array targeting Sox2-Klf4-Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG and U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RabV CVS-N2c(deltaG)-hChR2-YFP

   Plasmid

   #73465

   Purpose

   Expresses channelrhodopsin fused to YFP for monosynaptic circuit mapping and optogenetic activation

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   hChR2-YFP
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNP1 Aqua toehold switch

    Plasmid

    #107356

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 8 + Aquamarine
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159847

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

    Plasmid

    #207665

    Purpose

    Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

    Depositor

    Robert de Vries

    Article

    Spruit et al bioRxiv 2023.12.15.571923

    Insert

    Fucosidase E1_10125
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    amino acids 34-584 were used

    Promoter

    T7 promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK RagA(Q66L)-RagC

    Plasmid

    #99700

    Purpose

    Bacterial expression of RagA(Q66L)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dMASS_1

    Plasmid

    #209764

    Purpose

    Expression of dMASS_1 in mammalian cells under CMV promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

    Plasmid

    #159846

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

    Plasmid

    #159866

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

    Plasmid

    #159874

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid

    #182355

    Purpose

    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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