We narrowed to 16,548 results for: form
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Plasmid#72190PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.30
TagsFc-HisExpressionMammalianPromoterCMVAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH115gp160-QES.c14-754*
Plasmid#123282PurposeMammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (CH115) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL2 G116
Plasmid#123088PurposeGateway entry clone encoding human GABARAPL2 G116DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseGateway entry vector / entry cloneMutationDeleted amino acid 117. Stop codon after G116Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ncam1_1.0.1-AP-His
Plasmid#71961PurposeExpresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA
Plasmid#108773PurposeExpresses N-terminus to aa269 of VCre recombinase fused to GID1 CIDDepositorInsertVCre recombinase fragment N-terminus to aa269
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA
Plasmid#108774PurposeExpresses aa270 to C-terminus of VCre recombinase fused to GAI CIDDepositorInsertVCre recombinase fragment aa270 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-mATG5
Plasmid#58744PurposeExpresses /SECFP-ATG5 in mammalian cellsDepositorAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon3_2_Lb
Plasmid#155054PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 3
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC hKGA.wt V5-His IRES G418
Plasmid#110390Purposeγ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.DepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTags6xHis and V5ExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA
Plasmid#121799PurposepMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + CMV::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS
Plasmid#107323PurposeExpresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cellsDepositorInsertSpCas9-NmCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to nuclease dead NmCas9 …PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3A G120
Plasmid#123091PurposeExpresses 3xFLAG-LC3A G120 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
Tags3xFLAGExpressionMammalianMutationDeleted amino acid 121. Stop codon after G120.PromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
His10-B1 Integrin
Plasmid#186139PurposeFor expression of recombinant his10-beta1 integrin cytosolic domain in bacteriaDepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-hTERT-FF2
Plasmid#164910PurposeExpresses hTERT cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsHA tagAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Topo_SpCas9.tracr_LbCas12a.DR
Plasmid#155049PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
UseCRISPRAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TGmiR_Gb2-K
Plasmid#25766PurposeEntry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.DepositorAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-Tiam1
Plasmid#172102PurposeMammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)DepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR
Plasmid#121829PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/VPR (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB
Plasmid#121830PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/KRAB (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core
Plasmid#121832PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/p300core (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LK4-Ets2
Plasmid#44606DepositorAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3A G120
Plasmid#123071PurposeGateway entry clone encoding human MAP1LC3A G120DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseGateway entry vector / entry cloneMutationDeleted amino acid 121. Stop codon after G120.Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
hEBI3 pEF6-Flag (MJS_003)
Plasmid#72491PurposeExpression of human EBI3 in mammalian cells (Flag tag)DepositorAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng2.a-Fc-His
Plasmid#72120PurposeExpresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.b-Fc-His
Plasmid#72111PurposeExpresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA
Plasmid#108831PurposeExpresses N-terminus to aa396 of Flp recombinase fused to nMag CIDDepositorInsertFlp recombinase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ncam1_1.0.1-Fc-His
Plasmid#72087PurposeExpresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry
Plasmid#128014PurposeVector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4-DBD-3xFlag
UseGal4 entry vectorTagsGal4-DBD-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM S297A
Plasmid#169983PurposeTet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutationsDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only